نتائج البحث

DrosophilaBld10 Is a Centriolar Protein That Regulates Centriole, Basal Body, and Motile Cilium Assembly

• Authors : Timothy L. Megraw; Violaine Mottier-Pavie

Subjects: Cell Nucleus; Male; Neurons

• Source: Molecular Biology of the Cell. 20:2605-2614

تفاصيل العنوان

The regulation of mechanosensory motile cilium formation

• Authors : Andrew P. Jarman; Pleasantine Mill; P zur Lage

Subjects: 0303 health sciences; 03 medical and health sciences; Oral Presentation

• Source: Cilia

تفاصيل العنوان

Sensorium: The Original Raison D'etre of the Motile Cilium?

• Authors : Michel R. Leroux; Lynne M. Quarmby

Subjects: 0301 basic medicine; 03 medical and health sciences; Movement

• Source: Journal of Molecular Cell Biology. 2:65-67

تفاصيل العنوان

Impact of aquaporin-4 and CD11c + microglia in the development of ependymal cells in the aqueduct: inferences to hydrocephalus

• Authors : Francisco Mayo; Lourdes González-Vinceiro; Laura Hiraldo-González

Subjects: Pulmonary and Respiratory Medicine; Cell biology; Cilium

• Source: Fluids Barriers CNSFluids and Barriers of the CNS, Vol 21, Iss 1, Pp 1-23 (2024)

تفاصيل العنوان

The regulation of mechanosensory motile cilium formation

• Authors : Jarman, A; Lage, P; Mali, G

تفاصيل العنوان

CFAP300 mutation causing primary ciliary dyskinesia in Finland

• Authors : Rüdiger Schultz; Varpu Elenius; Mahmoud R. Fassad ...

Subjects: Pulmonary and Respiratory Medicine; 0301 basic medicine; Therapeutic Advances in Cystic Fibrosis Research

• Source: Front GenetFrontiers in Genetics, Vol 13 (2022)

تفاصيل العنوان

A case study for heat and mass transfer of viscous fluid flow in double layer due to ciliated channel

• Authors : Nahid Fatima; Kottakkaran Sooppy Nisar; Sania Shaheen

Subjects: Heat Transfer Enhancement in Nanofluids; Cell biology; Cilium

• Source: Case Studies in Thermal Engineering, Vol 45, Iss, Pp 102943-(2023)

تفاصيل العنوان

Figure 2. Electrical coupling of motile cilia to the cellular compartment. ; (A,B) Mean current-voltage relation recorded from the cell body (A, n = 6) or a motile cilium (B, n = 5) after break-in in aCSF (filled squares), subsequent cell uncoupling with flufenamic acid (FFA, 100 μM, 2 min, filled circles), or block of K+ conductances in TEA-Cl/BaCl2 (TEA/Ba2+, filled diamonds; only cell/cilium recordings with input resistance >1 GΩ after TEA-Cl/BaCl2 treatment are plotted). Holding potential between 200 ms steps, -80 mV. Note: The voltage in A and B refers to the command voltage. The voltage error, that is, the difference between the command voltage and membrane voltage produces a large error due to the high resistance through the cilium in series with the low, multicellular membrane resistance (resting K+ conductance, cell-cell connections via gap junctions). Thus, large currents are inaccurate: the top traces only serve to show that flufenamic acid uncouples cells. (C) Example capacitive currents recorded in response to a 20 mV hyperpolarizing voltage step (50 ms) for the cell body (black) and motile cilium (green) after uncoupling with flufenamic acid (FFA, 100 μM) and block of K+ conductances (TEA-Cl/BaCl2). The steady state (time-independent) current in the motile cilium trace is leak current. (D–F) Time constant (D), membrane capacitance (E), and series resistance (F) determined from an average of 100–200 sweeps of capacitive current for cell body (black squares) and motile cilium (green squares) recordings after cell uncoupling with flufenamic acid (FFA, 100 μM, ~5 min) and perfusion with TEA-Cl/BaCl2 (TEA/Ba2+, n = 7–8). (G) Resting membrane potential assessed under current clamp with a gramicidin-perforated patch for cell bodies (black squares) and motile cilia (green squares) before (n = 7 cell body, n = 8 motile cilia) and after (n = 7 cell body, n = 5 motile cilia) addition of flufenamic acid (FFA, 100 μM) to the bath. Open squares represent the range of individual cells/cilia; filled squares are the mean. Error bars; ± SEM. (H) Cartoon illustrating simplified equivalent circuit of access to the cellular compartment via a motile cilium. The cable-like properties of a motile cilium significantly increase the access (series) resistance. Block of gap junctions by flufenamic acid removes the contributions from neighboring cells.

تفاصيل العنوان

Figure 3—figure supplement 1. CaV-mediated currents and single channels in ependymal cells. ; (A) Reduction of CaV currents by nimodipine (10 μM, 2 min pre-incubation) and cadmium chloride (CdCl2, 100 μM) (n = 4, recorded from the cell body). Holding potential, -60 mV. Error bars; ± SEM. (B) Example of slightly distorted current observed in some whole-motile-cilium recordings due to the high series resistance. The red trace depicts the delayed opening of CaV channels as a result of the increased series resistance and delayed voltage clamp. Large oscillations indicate transient loss of voltage clamp. (C,D) Example of 5 consecutive traces recorded from a motile cilium in the cilium-attached configuration in presence of BayK8644 (5 μM, bath and pipette, C) and open point amplitude histogram of all recorded traces (D, fraction of full amplitude = 0.9). Single channel openings were observed in 1 of 29 recordings. Mean single channel amplitude was 1.2 pA.

تفاصيل العنوان

Figure 1. Ependymal motile cilia identification and patch clamp. ; (A) Immunolabeling of motile cilia in a section of the lateral ventricle from an Arl13B-EGFPtg mouse. Ciliary localization of Arl13B-EGFP was confirmed using anti-GFP (green) and anti-acetylated tubulin antibodies (red). (B) Anti-GFP (green) and anti-acetylated tubulin staining (red) of cultured ependymal cells at DIV10. GFP labeling of motile cilia varied, with some cells displaying only weak or barely detectable GFP fluorescence in cilia (arrows). Some cells were only sparsely ciliated (arrowhead). (C) Staining of cultured multiciliated cells with anti-GFP (green) and anti-Spag6 (red). (D) Representative staining of a previously recorded ependymal cell grown on a gridded glass bottom dish (grid size, 50 μm, arrow marks motile cilium). Motile cilia of sparsely ciliated cells were GFP (green) and Spag6 (red) positive (n = 40/45). Nuclei were labeled with Hoechst dye (blue, A–D). Panels B-D display average intensity z-projections of image stacks. Scale bars, 10 μm (A–C) and 5 μm (D). (E) Image showing dye diffusion into a motile cilium after successful break-in (50 μM Alexa 594 hydrazide, n = 9). Scale bar, 3 μm. (F) Example current (bottom) recorded in the whole-motile-cilium configuration in response to increasing voltage steps (top). Holding potential, -80 mV. (G) Mean steady state current after break-in plotted as a function of command voltage (n = 4). External solution (aCSF) with 3 mM KCl (black filled squares), 70 mM KCl (blue filled circles), and 140 mM KCl (red filled diamonds). Arrows in the graph indicate calculated EK values. Error bars; ± SEM.

تفاصيل العنوان