- Document Number:
20240389618
- Appl. No:
18/694132
- Application Filed:
September 27, 2022
- نبذة مختصرة :
A method for feeding invertebrates comprising: ▪providing a composition comprising a microbial organism or parts, extracts, oils or refinements thereof; and ▪administering the composition to invertebrates wherein the microbial organism is selected from the group consisting of Saccharomyces cerevisiae, Xanthophyllomyces sp., S. japonicus or Yarrowia Hpolytica; and wherein the microbial organism has been genetically modified to produce an invertebrate nutrient selected from the group consisting of 24-methylene cholesterol, isofucosterol, cholesterol or desmosterol.
- Claim:
1. A method for feeding invertebrates comprising: providing a composition comprising a microbial organism or parts, extracts, oils, mixtures or refinements thereof; and administering the composition to invertebrates, wherein the microbial organism is selected from the group consisting of Saccharomyces cerevisiae, Xanthophyllomyces sp., Schizosaccharomyces japonicus or Yarrowia lipolytica; and wherein the microbial organism has been genetically modified or selected to produce an invertebrate nutrient selected from the group consisting of cholesterol, desmosterol, 24-methylene cholesterol, and isofucosterol.
- Claim:
2. The method of claim 1, wherein the yeast has been modified or enhanced as compared to a parent microorganism to express a squalene-hopene cyclase enzyme or a squalene-tetrahymenol cyclase enzyme to enable growth under anoxic conditions.
- Claim:
3. The method of claim 1, wherein the invertebrate nutrient is produced under aerobic conditions.
- Claim:
4. The method of claim 1, wherein the microorganism has been further selected or modified not to express or produce invertebrate antinutrients.
- Claim:
5. The method of claim 1, wherein the invertebrate antinutrient is a sterol such as zymosterol or ergosterol.
- Claim:
6. The method of claim 1, wherein the composition is a whole invertebrate diet, part of an invertebrate diet or a dietary supplement.
- Claim:
7. The method of claim 1, wherein the composition is administered to invertebrates of the Apidae family, in particular honey bees or bumble bees.
- Claim:
8. The method of claim 1, wherein the composition is administered a. in solid form such as a patty or in liquid form such as a solution or spray; b. inside or outside the hive.
- Claim:
9. The method of claim 1, wherein there is a chemical, chromatographic or enzymatical modification step to obtain the invertebrate nutrient.
- Claim:
10. The method of claim 1, wherein the composition comprises: proteins in an amount from 10 w % to 50 w %, preferably of 20 w % to 40 w %, fatty acids in an amount from 1 w % to 20 w %, preferably of 2 w % to 12 w %, carbohydrates in an amount from 30 w % to 90 w %, preferably of 50 w % to 70 w %, optionally vitamins, and optionally minerals, wherein the total amount of components add up to 100 w % and wherein the w % are related to the total dry weight of the composition.
- Claim:
11. The method of claim 1, wherein the composition comprises an insect nutrient selected from then group consisting of cholesterol, desmosterol, 24-methylene cholesterol, and isofucosterol in an amount: From 0.01-5% of the diet as individual compounds, where no compound is ever less than 0.01% of the diet and from 0.1-5% of the diet in a mixture; or As a mixture, preferably from 0.1-1% and even more preferably from 0.3-0.5% for honey bees and 0.3-0.8% for bumble bees as a percentage of the total weight of the pollen substitute composition; and/or.
- Claim:
12. The method of claim 1, wherein the composition further comprises an insect nutrient including polyphenols in the following groups phenolic acids, proanthocyanidins, epicatechins, catechins, and flavonoids such as quercetin, naringenin, p-coumaric acid or mixtures thereof in an amount from 0.01-5% of the diet, preferably from 0.01-1% and even more preferably from 0.03-0.5% for honey bees and 0.03-0.8% for bumble bees as a percentage of the total weight of the pollen substitute composition and where the predominant class of phenolics is either proanthocyanidins or flavonoids and makes up at least 30% of the total polyphenols and preferably is 70-100% of the total polyphenols.
- Claim:
13. The method of claim 1, wherein the composition further comprises of an insect nutrient selected from the group consisting of carotenoids or xanthophylls such as b-carotene, lycopene, or astaxanthin or mixtures thereof in an amount from 0.01-5% of the diet, preferably from 0.01-1% and even more preferably from 0.03-0.5% for honey bees and 0.03-0.8% for bumble bees as a percentage of the total weight of the pollen substitute composition.
- Claim:
14. The method of claim 1, wherein the composition is essentially free of pollen.
- Claim:
15. Use of the composition of claim 1 for feeding invertebrates, in particular honey bees or bumble bees or stingless bees.
- Current International Class:
23; 23
- الرقم المعرف:
edspap.20240389618
Processing Request
No Comments.