Biological and Chemical Process Utilizing Chemoautotrophic Microorganisms for the Chemosynthetic Fixation of Carbon Dioxide and/or Other Inorganic Carbon Sources into Organic Compounds and the Generation of Additional Useful Products

20180179559

15/899303

February 19, 2018

The invention described herein presents compositions and methods for a multistep biological and chemical process for the capture and conversion of carbon dioxide and/or other forms of inorganic carbon into organic chemicals including biofuels or other useful industrial, chemical, pharmaceutical, or biomass products. One or more process steps utilizes chemoautotrophic microorganisms to fix inorganic carbon into organic compounds through chemosynthesis. An additional feature described are process steps whereby electron donors used for the chemosynthetic fixation of carbon are generated by chemical or electrochemical means, or are produced from inorganic or waste sources. An additional feature described are process steps for the recovery of useful chemicals produced by the carbon dioxide capture and conversion process, both from chemosynthetic reaction steps, as well as from non-biological reaction steps.

1.-26. (canceled)

27. A biological and chemical process for the capture and conversion of carbon dioxide and/or other sources of inorganic carbon, into organic compounds, comprising: introducing carbon dioxide gas, either alone and/or dissolved in a mixture or solution further comprising carbonate ion and/or bicarbonate ion, and/or introducing inorganic carbon contained in a solid phase into an environment suitable for maintaining chemoautotrophic organisms and/or chemoautotroph cell extracts; and fixing the carbon dioxide and/or inorganic carbon into organic compounds within the environment via at least one chemosynthetic carbon fixing reaction utilizing obligate and/or facultative chemoautotrophic microorganisms; wherein where the chemosynthetic carbon fixing reaction is driven by chemical and/or electrochemical energy provided by electron donors and electron acceptors that have been generated chemically and/or electrochemically and/or or are introduced into the environment from at least one source external to the environment, wherein biomass and/or biochemicals are produced by the at least one chemosynthetic reaction, and wherein the biomass and/or biochemicals are separated from the environment and are processed into a product comprising an animal feed, a fertilizer, a soil additive, a soil stabilizer, a carbon source for large scale fermentations, and/or a nutrient source for the growth of other microbes or organisms.

28. A process according to claim 27, wherein the animal feed product is a feed for cattle, sheep, chickens, pigs, and/or fish.

29. A process according to claim 27, wherein said biomass and/or biochemicals are processed into a carbon source for a large scale fermentation and/or a nutrient source for the growth of other microbes or organisms, wherein said large scale fermentation and/or said other microbes or organisms produce one or more of: commercial enzymes, antibiotics, amino acids, vitamins, and bioplastics.

30. A process according to claim 27, wherein said biomass and/or biochemicals are processed into a nutrient source for the growth of fish.

31. A process according to claim 27, wherein molecular hydrogen acts as an electron donor.

32. A process according to claim 31, wherein said hydrogen is generated through electrolysis of water and/or thermochemical splitting of water.

33. A process according to claim 32, wherein said electrolysis of water comprises at least one of: Proton Exchange Membranes (PEM); a liquid electrolyte; high-pressure electrolysis; and high temperature electrolysis of steam (HTES).

34. A process according to claim 33, wherein said electrolyte comprises potassium hydroxide.

35. A process according to claim 32, wherein said thermochemical splitting of water comprises at least one of: iron oxide cycle; cerium(IV) oxide-cerium(III) oxide cycle; zinc zinc-oxide cycle; sulfur-iodine cycle; copper-chlorine cycle; calcium-bromine-iron cycle; and hybrid sulfur cycle.

36. A process according to claim 31, wherein said hydrogen is generated through one or more of: electrolysis of hydrogen sulfide; thermochemical splitting of hydrogen sulfide; and the half-cell reduction of H+ to H2 accompanied by the half-cell oxidization of electron sources comprising ferrous iron (Fe2+) oxidized to ferric iron (Fe3+ and/or the oxidation of sulfur compounds, wherein the oxidized iron or sulfur is recycled to back to a reduced state through additional chemical reactions with minerals comprising at least one of a metal sulfide, hydrogen sulfide, and a hydrocarbon.

37. A process according to claim 31, wherein said hydrogen is generated through an electrochemical or thermochemical process known to produce hydrogen with low- or no-carbon dioxide emissions, comprising at least one of: carbon capture and sequestration enabled methane reforming; carbon capture and sequestration enabled coal gasification; the Kværner process; a process that generates a carbon-black product; and carbon capture and sequestration enabled gasification or pyrolysis of biomass.

38. A process according to claim 27, wherein said electron donors and/or electron acceptors are generated or recycled using renewable, alternative, or conventional sources of power that are low in greenhouse gas emissions, and wherein said sources of power comprise at least one of: photovoltaic, solar thermal, wind, hydroelectric, nuclear, geothermal, enhanced geothermal, ocean thermal, ocean wave, and tidal power sources.

39. A process according to claim 27, wherein said electron donor comprises one or more of: ammonia; ammonium; carbon monoxide; dithionite; elemental sulfur; a hydrocarbon; hydrogen; a sulfide; a sulfite; a thionate; a thionite; a transition metal and/or its sulfide; an oxide; a chalcogenide; a halide; a hydroside; an oxyhydroxide; a phosphate; a sulfate; a carbonate; and a conduction or valence band electron in a solid state electrode material.

40. A process according to claim 27, wherein said electron donor is generate within or recycled to the environment through non- or low-carbon dioxide emitting chemical reactions with hydrocarbons, comprising one or more of: thermochemical reduction of sulfate reaction (TSR); the Muller-Kuhne reaction for the production of hydrogen sulfide or reduced sulfur; and methane reforming-like reactions utilizing metal oxides in place of water, wherein the metal oxides comprise one or more of: iron oxide, calcium oxide, and magnesium oxide, and wherein the hydrocarbon is reacted to form solid carbonate with little or no emission of carbon dioxide gas along with hydrogen electron donor product.

41. A process according to claim 27, wherein said electron acceptor comprises one or more of: carbon dioxide; oxygen; a nitrite; a nitrate; a transition metal ion; a sulfate; and a valence or conduction band hole in a solid state electrode material.

42. A process according to claim 27, wherein the fixing step is followed by one or more process steps in which unused nutrients and/or process water left after removal of chemoautotrophic cell mass and/or chemical co-products of chemosynthesis and/or waste products or contaminants of the process stream produced during the fixing step are recycled back into a reactor system in which the chemosynthetic carbon fixing reaction is performed to support further chemosynthesis.

43. A process according to claim 27, wherein the chemoautotrophic microorganisms comprise one or more of: Acetoanaerobium sp.; Acetobacterium sp.; Acetogenium sp.; Achromobacter sp.; Acidianus sp.; Acinetobacter sp.; Actinomadura sp.; Aeromonas sp.; Alcaligenes sp.; Arcobacter sp.; Aureobacterium sp.; Bacillus sp.; Beggiatoa sp.; Butyribacyerium sp.; Carboxydothermus sp.; Clostridium sp.; Comamonas sp.; Dehalobacter sp.; Dehalococcoides sp.; Dehalosprillum sp.; Desulfobacterium sp.; Desulfomonile sp.; Desulfotomaculum sp.; Desulfovibrio sp.; Desulfurosarcina sp.; Ectothiorhodospira sp.; Enterobacter sp.; Eubacterium sp.; Ferroplasma sp.; Halothibacillus sp.; Hydrogenbacter sp.; Hydrogenomonas sp.; Leptospirillum sp.; Metallosphaera sp.; Methanobacterium sp.; Methanobrevibacter sp.; Methanococcus sp.; Methanosarcina sp.; Micrococcus sp.; Nitrobacter sp.; Nitrosococcus sp.; Nitrosolobus sp.; Nitrosomonas sp.; Nitrosospira sp.; Nitrosovibrio sp.; Nitrospina sp.; Oleomonas sp.; Paracoccus sp.; Peptostreptococcus sp.; Planctomycetes sp.; Pseudomonas sp.; Ralstonia sp.; Rhodobacter sp.; Rhodococcus sp.; Rhodocyclus sp.; Rhodomicrobium sp.; Rhodopseudomonas sp.; Rhodospirillum sp.; Shewanella sp.; Streptomyces sp.; Sulfobacillus sp.; Sulfolobus sp.; Thiobacillus sp.; Thiomicrospira sp.; Thioploca sp.; Thiosphaera sp.; Thiothrix sp.; sulfur-oxidizer; hydrogen-oxidizers; iron-oxidizers; acetogens; methanogens; consortiums of microorganism that include chemoautotrophs; chemoautotrophs native to at least one of hydrothermal vents, geothermal vents, hot springs, cold seeps, underground aquifers, salt lakes, saline formations, mines, acid mine drainage, mine tailings, oil wells, refinery wastewater, coal seams, deep sub-surface, waste water and sewage treatment plants, geothermal power plants, sulfatara fields, and soils; and extremophiles selected from one or more of thermophiles, hyperthermophiles, acidophiles, halophiles, and psychrophiles.

44. A process according to claim 27, wherein said at least one chemosynthetic carbon fixing reaction is performed by chemoautotrophic microorganisms that have been improved, optimized or engineered for the fixation of carbon dioxide and/or other forms of inorganic carbon and the production of organic compounds.

45. A process according to claim 27, wherein said electron donor is generated from pollutants or waste products selected from one or more of: process gas; tail gas; enhanced oil recovery vent gas; biogas; acid mine drainage; landfill leachate; landfill gas; geothermal gas; geothermal sludge or brine; metal contaminants; gangue; tailings; sulfides; disulfides; mercaptans selected from one or more of methyl mercaptan, dimethyl mercaptan, and ethyl mercaptan; carbonyl sulfide; carbon disulfide; alkanesulfonates dialkyl sulfides; thiosulfate; thiofurans; thiocyanates; isothiocyanates; thioureas; thiols; thiophenols; thioethers; thiophene; dibenzothiophene; tetrathionate; dithioite; thionate; dialkyl disulfides; sulfones; sulfoxides; sulfolanes; sulfonic acid; dimethylsulfoniopropionate; sulfonic esters; hydrogen sulfide; sulfate esters; organic sulfur; and sour gases.

46. A process according to claim 27, wherein delivery of reducing equivalents from the electron donor to the chemoautotrophic microorganisms for the chemosynthetic reaction during the fixing step is kinetically and/or thermodynamically enhanced by one or more of: introduction of hydrogen storage materials into the environment in the form of a solid support media for microbial growth that facilitates bringing absorbed or adsorbed hydrogen electron donors into close proximity with the chemoautotrophic organisms; introduction of electron mediators comprising one or more of: cytochromes, formate methyl-viologen, NAD+/NADH, neutral red (NR), and quinones to help transfer reducing power from a poorly soluble electron donor comprising H2 gas or electrons in solid state electrode materials into chemoautotrophic culture media in the environment; and introduction of electrode materials in the form of a solid growth support media directly into the environment to facilitate bringing solid state electrons into close proximity with the chemoautotrophic microorganisms.

47. A process according to claim 27, wherein said environment comprises a bioreactor, and wherein said microorganisms are maintained in a culture medium in said bioreactor.

48. A process according to claim 47, wherein said bioreactor is formed at least in part by a microbial culture apparatus selected from: an airlift reactor; a biological scrubber column; a bubble column; a continuous stirred tank reactor; a counter-current, upflow, expanded-bed reactor; a digestor for a sewage and/or waste water treatment or bioremediation system; one or more filters; a fluidized bed reactor; a gas lift fermenter; an immobilized cell reactor; a membrane biofilm reactor; a mine shaft; a Pachuca tank; a packed-bed reactor; a plug-flow reactor; a static mixer; a tank; a trickle bed reactor; a vat; and/or a vertical shaft bioreactor.

49. A process according to claim 27, further comprising prior to the fixing step, a step of reacting carbon dioxide with minerals to form a carbonate or bicarbonate product, which is then used in the fixing step.

50. A process according to claim 27, comprising fixing the carbon dioxide and/or inorganic carbon into the organic compounds via at least one chemosynthetic carbon fixing reaction within a reactor system, wherein the electron donor utilized in the chemosynthetic carbon fixing reaction is produced via a non-biological process in the reactor system.

51. A process according to claim 27, wherein the chemosynthetic microorganisms are obligate anaerobes.

52. A process for the capture and conversion of carbon dioxide and/or other sources of inorganic carbon, into organic compounds, comprising: introducing a carbon source in the form of flue gas comprising carbon dioxide and/or in the form of an aqueous solution comprising inorganic carbon into an environment in a bioreactor that is suitable for maintaining chemoautotrophic microorganisms; introducing an electron donor that is separate from the carbon source into the environment in the bioreactor; fixing the carbon dioxide in the flue gas and/or inorganic carbon in the aqueous solution into the organic compounds within the environment in the bioreactor via at least one chemosynthetic carbon fixing reaction utilizing chemoautotrophic microorganisms and using at least one electron donor and at least one electron acceptor; and wherein said electron donor and/or said electron acceptor are generated and/or refined from at least one inorganic chemical, wherein said electron donor is generated separately from the carbon source and externally to the bioreactor using a renewable, alternative, or low CO2 emission power source, wherein said electron donor is molecular hydrogen that is generated using said power source, through electrolysis of water, wherein biomass and/or biochemicals are produced by the at least one chemosynthetic reaction, and wherein the biomass and/or biochemicals are separated from the environment and are processed into a product comprising an animal feed, a fertilizer, a soil additive, a soil stabilizer, a carbon source for large scale fermentations, and/or a nutrient source for the growth of other microbes or organisms.

53. A process according to claim 52, wherein the process for capture and conversion of carbon dioxide or inorganic carbon results in a net reduction of gaseous CO2 released to the atmosphere.

54. A process according to claim 52, wherein the animal feed product is a feed for cattle, sheep, chickens, pigs, and/or fish.

55. A process according to claim 52, wherein said biomass and/or biochemicals are processed into a carbon source for a large scale fermentation and/or a nutrient source for the growth of other microbes or organisms, wherein said large scale fermentation and/or said other microbes or organisms produce one or more of: commercial enzymes, antibiotics, amino acids, vitamins, and bioplastics.

56. A process according to claim 52, wherein said biomass and/or biochemicals are processed into a nutrient source for the growth of fish.

12; 12; 12

edspap.20180179559