- Document Number:
20170356019
- Appl. No:
15/601157
- Application Filed:
May 22, 2017
- نبذة مختصرة :
Provided are methods and/or systems having advantages of cost effective, time saving, and informative user-friendly characteristics to accomplish trait introgression. The methods provided comprise determining presence of omega-3 fatty acids (for example docosahexanoic acid or DHA; docosapentaenoic acid or DPA; Alpha linolenic acid or ALA; and eicosapentaenoic acid or EPA) using Fourier Transformed Infra Red (FTIR) spectrum. The use of FTIR enables analysis of the oil contained in the seeds using a multivariate-based Mid-FTIR model. The methods and/or systems provided advantages of non-destructive analysis to provide information to facilitate trait introgression and other breeding applications.
- Assignees:
Dow AgroSciences LLC (Indianapolis, IN, US)
- Claim:
1. A method for determining presence of poly unsaturated fatty acids (PUFAs) in a plant part, comprising, (a) obtaining a sample of the plant part; (b) performing a Fourier Transform Infra Red (FTIR) spectroscopy comprising a first absorption band between about 3800 cm−1 wavenumber and about 2600 cm−1 wavenumber; (c) comparing the FTIR spectroscopy result of step (b) to existing reference FTIR spectroscopy results; and (d) predicting whether the sample containing at least one PUFA.
- Claim:
2. The method of claim 1, further comprising a second absorption band between about 7400 cm−1 wavenumber and about 5400 cm−1 wavenumber.
- Claim:
3. The method of claim 1, wherein the plant part comprises a seed.
- Claim:
4. The method of claim 1, wherein the plant part comprises a canola seed.
- Claim:
5. The method of claim 1, wherein the PUFA comprises an omega-3 poly unsaturated fatty acid.
- Claim:
6. The method of claim 5, wherein the omega-3 poly unsaturated fatty acid is selected from the group consisting of docosahexanoic acid (DHA), docosapentaenoic acid (DPA), alpha linolenic acid (ALA), eicosapentaenoic acid (EPA), and combinations thereof.
- Claim:
7. The method of claim 1, wherein the plant is selected from the group consisting of canola, soybean, sunflower, cotton, Brassica, rapeseed, peanut, corn, wheat, rice, alfalfa, and oat.
- Claim:
8. The method of claim 1, wherein the plant is a transgenic plant.
- Claim:
9. The method of claim 1, wherein the plant part comprises a transgenic plant cell.
- Claim:
10. The method of claim 1, wherein the plant is a back-crossed plant of a first generation, second generation, third generation, fourth generation, fifth generation back-crossing plant, or combinations thereof.
- Claim:
11. A computerized system for plant trait introgression, comprising, (a) a database comprising existing reference Fourier Transform Infra Red (FTIR) spectroscopy results for samples containing at least one poly unsaturated fatty acid (PUFA) and for samples containing no PUFA; (b) at least one sample of a plant part; (c) a customized sample holder made of non-reflective metal alloy; and (d) an instrument capable of measuring FTIR spectroscopy.
- Claim:
12. The system of claim 11, wherein the FTIR spectroscopy results comprise a first absorption band between about 3800 cm−1 wavenumber and about 2600 cm−1 wavenumber.
- Claim:
13. The system of claim 12, wherein the FTIR spectroscopy results further comprise a second absorption band between about 7400 cm−1 wavenumber and about 5400 cm−1 wavenumber.
- Claim:
14. The system of claim 11, wherein the plant part comprises a seed.
- Claim:
15. The system of claim 11, wherein the plant part comprises a canola seed.
- Claim:
16. The system of claim 11, wherein the PUFA comprises an omega-3 poly unsaturated fatty acid.
- Claim:
17. The system of claim 14, wherein the omega-3 poly unsaturated fatty acid is selected from the group consisting of docosahexanoic acid (DHA), docosapentaenoic acid (DPA), alpha linolenic acid (ALA), eicosapentaenoic acid (EPA), and combinations thereof.
- Claim:
18. The system of claim 11, wherein the plant is selected from the group consisting of canola, soybean, sunflower, cotton, Brassica, rapeseed, peanut, corn, wheat, rice, alfalfa, and oat.
- Claim:
19. The system of claim 11, wherein the plant is a transgenic plant.
- Claim:
20. The system of claim 11, wherein the plant part comprises a transgenic plant cell.
- Claim:
21. A method of accelerating introgression of transgenic plants and/or eliminating gene silencing of transgenic plants, comprising, (a) obtaining Fourier Transform Infra Red (FTIR) spectroscopy information from a plant part for a trait of interest; (b) selecting seeds from the plant providing the plant part and expressing said trait based on the FTIR results from Step (a); (c) planting the seeds from Step (b); (d) collecting progeny seeds from Step (c); and repeating steps (a)-(d) for at least three generations.
- Claim:
22. The method of claim 21, wherein the plant part comprises a seed.
- Claim:
23. The method of claim 21, wherein the trait of interest comprises presence of at least one poly unsaturated fatty acid (PUFA).
- Claim:
24. The method of claim 21, wherein the PUFA comprises an omega-3 poly unsaturated fatty acid.
- Claim:
25. The system of claim 14, wherein the omega-3 poly unsaturated fatty acid is selected from the group consisting of docosahexanoic acid (DHA), docosapentaenoic acid (DPA), alpha linolenic acid (ALA), eicosapentaenoic acid (EPA), and combinations thereof.
- Current International Class:
12; 23; 01; 12
- الرقم المعرف:
edspap.20170356019
Processing Request
No Comments.