Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Impact of heat shock protein 90 inhibition on the proteomic profile of lung adenocarcinoma as measured by two-dimensional electrophoresis coupled with mass spectrometry

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Publisher Information:
      Multidisciplinary Digital Publishing Institute 2019
    • نبذة مختصرة :
      Heat shock protein 90 (HSP90) is an important chaperone in lung adenocarcinoma, with relevant protein drivers such as EGFR (epidermal growth factor receptor) and EML4-ALK (echinoderm microtubule-associated protein-like protein4 fused to anaplastic lymphoma kinase) depending on it for their correct function, therefore HSP90 inhibitors show promise as potential treatments for lung adenocarcinoma. To study responses to its inhibition, HSP90 was pharmacologically interrupted by geldanamycin and resorcinol derivatives or with combined inhibition of HSP90 plus HSP70 in lung adenocarcinoma cell lines. Two-dimensional electrophoresis was performed to identify proteomic profiles associated with inhibition which will help to understand the biological basis for the responses. HSP90 inhibition resulted in altered protein profiles that differed according the treatment condition studied. Results revealed 254 differentially expressed proteins after treatments, among which, eukaryotic translation initiation factor3 subunit I (eIF3i) and citrate synthase demonstrated their potential role as response biomarkers. The differentially expressed proteins also enabled signalling pathways involved in responses to be identified; these included apoptosis, serine-glycine biosynthesis and tricarboxylic acid cycle. The proteomic profiles identified here contribute to an improved understanding of HSP90 inhibition and open possibilities for the detection of potential response biomarkers which will be essential to maximize treatment efficacy in lung adenocarcinoma.
    • الموضوع:
      Lung cancer; Proteomics; Chaperones; HSP90 inhibitors; artículo
    • Availability:
      Open access content. Open access content
      https://creativecommons.org/licenses/by/4.0
      openAccess
    • Other Numbers:
      CTK oai:digital.csic.es:10261/212677
      Cells 8(8): 806 (2019)
      10.3390/cells8080806
      31370342
      1286548406
    • Contributing Source:
      CSIC
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1286548406
HoldingsOnline