Structure, physicochemical properties, and hypolipidemic activity of soluble dietary fiber obtained from button mushroom (Agaricus bisporus)

The purpose of this paper was to investigate the structural characteristics, physicochemical properties, and hypolipidemic activity of soluble dietary fiber from button mushroom (BMSDF). Our results show that BMSDF is mainly composed of galactose, glucose and mannose, has the typical spectral characteristics of dietary fiber (FT-IR), characteristic diffraction curves (XRD), and the typical characteristics of polysaccharide nuclear magnetic hydrogen spectrum, which contained both α and β glycosidic bonds. The loose and porous microstructure of BMSDF observed under scanning electron microscopy makes it has good water holding capacity, oil holding capacity, cholesterol adsorption capacity, bile salt binding capacity. The lipid metabolism-related enzymes inhibitory assay results showed that 1.2 mg/mL BMSDF reached 75.29 % of the effect of orlistat and the inhibition of cholesterol esterase reached 37.05 %. And 100 μg/mL BMSDF reduces lipid deposition, significantly decreases the content of triglyceride and serum total cholesterol in oleic acid-induced HepG2 high-fat cells, while improves abnormal liver function enzyme activity, exhibiting good hypolipidemic biological activity.