نبذة مختصرة : Abstract Biopesticides have shown high potential in controlling insect pests, including Diatraea saccharalis Fabr. (Lepidoptera: Crambidae). From that perspective, this study evaluated lethal concentrations of the product Fitoneem®, which has Azadirachtin A and B, and its impact on the mortality pattern of D. saccharalis. Fitoneem® was diluted in distilled water at concentrations of 5000 ppm, 10000 ppm, 20000 ppm, 30000 ppm, and 50000 ppm. The larvae were exposed to the product through the following exposure routes: (1) topical spray, applying the product directly to the larvae's prothorax; (2) ingestion, exposing the larvae through inoculation of the product in the artificial diet; and (3) spraying the entire body of the insect, using a manual spray bottle. After exposure, mortality was assessed at 24 intervals for six consecutive days. According to the concentrations studied of Fitoneem®, larvae exposed via spraying all over the body showed LC50 of 18500 ppm and LC90 of 55100 ppm; via topical application, the LC50 was 15700 ppm and LC90 of 17400 ppm, and via ingestion, LC50 of 22500 ppm and LC90 of 70400 ppm. The median lethal time ranged from 96 to 144 hours. Therefore, Fitoneem® in low concentrations promotes high mortality levels in D. saccharalis. Hence, our results show the potential of using Azadirachtin A and B to manage this crucial pest in sugarcane and information that can contribute to understanding its action mode in the insect.
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