نبذة مختصرة : Background: The development of materials capable of regenerating damaged tissues and their implementation in medical practice is a promising direction in tissue engineering and regenerative medicine.Objective: To investigate physicomechanical and chemical properties of marine collagen-based matrices for tissue engineering through in vitro experiments.Materials and methods: The study materials included two experimental marine collagen-based matrices: one was produced using 10% glutaraldehyde as a cross-linking agent (Group 1), while 10% glyoxal was added during the production of the second sample (Group 2). A bovine collagen-based matrix served as the control (Group 3). The deformation degree, sorption capacity, adhesion degree, and pH were determined. Statistical analysis was performed using Statistica 13.0 Pro (Dell Software, USA).Results: The deformation degree in group 3 was 8.11% lower than in group 1 (Р = .99) and 31.88% lower than in group 2 (Р = .0053). The sorption capacity (mass) in group 3 was 7.2 times higher than in group 1 (Р = .0144) and 18.22 times higher than in group 2 (Р = .0001). The sorption capacity (volume) in group 3 was 4 times higher than in group 1 (Р = .0102) and 7.88 times higher than in group 2 (Р = .0001). The adhesion degree in group 1 was 1.32 times higher than in group 2 (Р ≥ .05) and 1.09 times higher than in group 3 (Р ≥ .05). The pH level in group 3 was 1.12 times higher than in group 1 (Р ≥ .05) and 1.17 times higher than in group 2 (Р = .0383).Conclusions: The performance analysis revealed numerous shortcomings of the pilot samples with 10% glyoxal, warranting their exclusion from further studies. The samples with 10% glutaraldehyde demonstrated comparable or superior performance to the control group.
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