Development of a serological test for the detection of anti-darbepoetin antibodies in cats ; Mise au point d'un test sérologique de détection des anticorps anti-darbépoétine chez le chat

Many cats suffer from chronic kidney disease, most of them also suffer from chronic anemia. In fact, the kidney is by far the main organ responsible for the synthesis of erythropoietin in the body, a major erythrocyte growth factor. With the aim of improving the quality of life of cats suffering from chronic anemia, a drug from the human pharmacopoei has begun to be used in cats. However, although the well-tolerated and highly effective in humans, clinicians observe frequent therapeutic failures in cats treated with this drug. This is darbepoetin, an erythropoietin synthesized from the protein sequence of human erythropoietin. However, darbepoetin has only 82% sequence homology with feline endogenous erythropoietin compared with 95% for human endogenous erythropoietin. We therefore hypothesized the presence of an immunogenic reaction mediated by antibodies to this human drug in the feline species. We have succeeded in developing an ELISA model in which we appear to have detected the presence of these anti-darbepoetin antibodies in two out of three cats tested. It would therefore appear that anti-darbepoetin antibodies do exist in cats. However, further still needs to be carried out with a substantial number of samples, in order to number of samples, in order to determine the statistical link between the presence between the presence of these anti-darbepoetin antibodies and the efficacy of darbepoetin treatment in cats. In the future, the aim is to create a rapid ELISA test based on the same model as the one we have developed, for routine use by clinicians directly at the patient's bedside. ; De nombreux chats sont atteints de maladie rénale chronique, la majorité d'entre eux souffrant également d'anémie chronique. En effet, le rein est de loin le principal organe responsable de la synthèse de l'érythropoïétine au sein de l'organisme, un facteur de croissance majeur des érythrocytes. Dans l'objectif d'une amélioration de la qualité de vie des chats souffrant d'anémie chronique, un médicament provenant de la ...