Veterinarinių antibiotikų poveikis gėlavandeniams vėžiagyviams / ; Effect of veterinary antibiotics on freshwater crustaceans.

VILNIUS UNIVERSITY LIFE SCIENCES CENTER Lukas Kežunas Bachelor's Thesis EFFECT OF VETERINARY ANTIBIOTICS ON FRESHWATER CRUSTACEANs SUMMARY One of the largest groups of freshwater pollutants are pharmaceutical substances. They are used globally and in large quantities. Medications, their residues, and products enter surface waters through wastewater systems or aquaculture. A careless approach to their exploitation and safe disposal has dire consequences: water sources become contaminated, bacterial resistance to these drugs increases, and toxic effects are caused to aquatic life. For this ecotoxicological test, we chose three different, commonly used, and frequently encountered in water antibiotics: sulfamethoxazole, tetracycline, and ciprofloxacin. The study was conducted on Ceriodaphnia dubia crustaceans. The aim of this work is to determine the chronic impact of veterinary antibiotics: sulfamethoxazole, ciprofloxacin, and tetracycline on the viability, reproduction, and growth of Ceriodaphnia dubia over three generations. A concentration of 0.1 mg/L of ciprofloxacin had a negative impact on the survival of Ceriodaphnia dubia over 21 days in all three generations, and concentrations of 0.03 and 3 mg/L of tetracycline had a negative impact in the F2 and F3 generations. The three tested concentrations of sulfamethoxazole (0.1, 1, 5 mg/L) had no impact on Ceriodaphnia dubia survival over three generations. Ciprofloxacin inhibited Ceriodaphnia dubia reproduction in all tested concentrations, with the strongest effect observed in the F1 generation. The inhibition of reproduction in daphnids affected by sulfamethoxazole was directly dependent on the concentration, but there was no difference between generations. Tetracycline inhibited daphnid reproduction in all three generations. Sulfamethoxazole had no effect on Ceriodaphnia dubia growth over three generations. A concentration of 0.1 mg/L of ciprofloxacin negatively affected growth. Crustaceans exposed to tetracycline in the F1 and F2 generations produced larger ...