Analysis of the morphology and the cell cycle of L. infantum S9F1 and L. mexicana T7 Cas9.

(A) Different morphological patterns observed in exponentially growing L. infantum S9F1 vs. L. mexicana T7 Cas9 promastigote forms by microscopy. (K) represents the kinetoplast, (N) the nucleus, and (F) the flagellum. (B) NK pattern analysis of L. infantum S9F1 vs. L. mexicana T7 Cas9 promastigote forms. The data were obtained from 600 cells counted from three independent experiments. Statistical test used: Multiple t-test (two-sided), ns: not significant. (C) Comparison of the flagellum length of L. infantum S9F1 vs. L. mexicana T7 Cas9 promastigote forms in exponential and stationary phases. (left); of the cell body length of L. infantum S9F1 vs L. mexicana T7 Cas9 in exponential and stationary phases (right). Measurements were done using ImageJ. One-way ANOVA followed by Tukey’s test was used to compare flagellum length and cell body length. Significance levels: **** p-value < 0.0001, ***p-value<0.001 and *p-value<0.05.