نبذة مختصرة : All the datasets and scripts have been uploaded on the open-source CIRAD dataverse. These data can be accessed using the links provided: BPO-59: https://doi.org/10.18167/DVN1/9K4J0D. BPO-60: https://doi.org/10.18167/DVN1/5LFLWU. BPO-64: https://doi.org/10.18167/DVN1/WJPM4N. BPO-66: https://doi.org/10.18167/DVN1/TPDEX8. GWT-1: https://doi.org/10.18167/DVN1/PNUURS. GWT-2: https://doi.org/10.18167/DVN1/XIOTAO. GWT-3: https://doi.org/10.18167/DVN1/EVHGJO. GWT-4: https://doi.org/10.18167/DVN1/TYWQ0R. GWT-5: https://doi.org/10.18167/DVN1/0JUVIK. GWT-6: https://doi.org/10.18167/DVN1/IHRVQS. GWT-7: https://doi.org/10.18167/DVN1/XF2A3E. GWT-9: https://doi.org/10.18167/DVN1/9IHYTJ. GWT-10: https://doi.org/10.18167/DVN1/JHKGIO. GWT-11: https://doi.org/10.18167/DVN1/LB5HTS. GWT-12: https://doi.org/10.18167/DVN1/YKFVR1. GWT-13: https://doi.org/10.18167/DVN1/59AVAI. GWT-14: https://doi.org/10.18167/DVN1/ZNXDNL. GWT-15: https://doi.org/10.18167/DVN1/NPRFXF. GWT-16: https://doi.org/10.18167/DVN1/IX2M3J. GWT-17: https://doi.org/10.18167/DVN1/HPQU43. GWT-18: https://doi.org/10.18167/DVN1/L4LBY7. GWT-19: https://doi.org/10.18167/DVN1/YTRCBN. Scripts for RNA-Seq and sRNA analyses: https://doi.org/10.18167/DVN1/K1OKGY. ; International audience ; . Endogenous banana streak virus (eBSV) integrants derived from three distinct species, present in Musa balbisiana (B) but not Musa acuminata (A) banana genomes are able to reconstitute functional episomal viruses causing banana streak disease in interspecific triploid AAB banana hybrids but not in the diploid (BB) parent line, which harbours identical eBSV loci. Here, we investigated the regulation of these eBSV. In-depth characterization of siRNAs, transcripts and methylation derived from eBSV using Illumina and bisulfite sequencing were carried out on eBSV-free Musa acuminata AAA plants and BB or AAB banana plants with eBSV. eBSV loci produce low-abundance transcripts covering most of the viral sequence and generate predominantly 24-nt siRNAs. siRNA accumulation is restricted to ...
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