نبذة مختصرة : Lab-on-a-chip (LoC) are microfluidic devices that are increasingly used in biomedical and environmental analysis. It is expected that millions of these devices will be used daily in hospitals and homes in the coming years. However, they are mainly single-use devices, and most of them are manufactured from hydrocarbons (thermoplastics or PDMS), resulting in significant pollution due to their production and disposal, either by incineration or by being dumped in open-air landfill sites. To offer an eco-responsible alternative, we have developed a fabrication process for microfluidic devices from chitosan, a bio-sourced, biodegradable, and biocompatible polysaccharide. We compared several strategies to obtain transparent and thick films from chitosan powder, that minimizes their swelling in aqueous solution. Furthermore, their mechanical and physical properties (rugosity, hydrophobicity, Young’s modulus, hardness) were characterized. Both hot-embossing and micro-drilling were investigated to produce microchannels on the resulting chitosan films. Then, an adhesion step based on the deposition of a dry photoresist on micro-structured chitosan films enabled their bonding to either a glass slide or another chitosan film. We were then able to prepare the first functional chitosan microfluidic devices. Their behaviors (range of flowrates and duration, diffusion of molecules, channel deformation) during injection were examined. Finally, cells (ML-2 or MDA-MB-231) were successfully cultivated on chitosan films. This led to the implementation in a chitosan microfluidic device of a cell culture chamber allowing the observation over a few days of their behavior. This study paves the way for the development of eco-friendly lab-on-a-chip integrating various functions. ; Les laboratoires sur puce (LoC) sont des dispositifs microfluidiques de plus en plus utilisés dans les domaines de l’analyse biomédicale ou environnementale. Il est estimé que des millions de ces dispositifs seront utilisés quotidiennement dans les hôpitaux et ...
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