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One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • الموضوع:
      2024
    • Collection:
      Discovery - University of Dundee Online Publications
    • نبذة مختصرة :
      Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.
    • File Description:
      application/pdf
    • ISSN:
      2666-1667
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/38460134; info:eu-repo/semantics/altIdentifier/pissn/2666-1667; info:eu-repo/semantics/altIdentifier/eissn/2666-1667
    • الرقم المعرف:
      10.1016/j.xpro.2024.102929
    • الدخول الالكتروني :
      https://discovery.dundee.ac.uk/en/publications/d0c62175-8498-417b-92e0-e85c5945adbe
      https://doi.org/10.1016/j.xpro.2024.102929
      https://discovery.dundee.ac.uk/ws/files/122532099/1-s2.0-S2666166724000947-main.pdf
      https://www.scopus.com/pages/publications/85187001165
    • Rights:
      info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0/
    • الرقم المعرف:
      edsbas.5F428F04