Figure 1. Identification of neuronal populations required for yeast feeding. ; (A) Number of sips from 10% yeast (left) and 20 mM sucrose (right) by mated female flies of the genotype Ir76b-GAL4,UAS-GCaMP6s, fed for 3 days on a holidic diet with the indicated composition. (B) Yeast preference index (YPI) of yeast-deprived female flies in which 1261-GAL4 neurons were acutely silenced and corresponding controls. (C) Expression pattern of 1261-GAL4 in the head and tarsus of the fly. Boxes are magnifications of the antenna or the labellum. Note absence of signal in the maxillary palps. Green represents GFP signal and gray the cuticular autofluorescence. (D) YPI of yeast-deprived female flies in which Ir76b- or Ir25a-GAL4 neurons were acutely silenced and corresponding controls. (E) YPI of yeast-deprived female flies in which 1261-GAL4 neurons were all silenced, or with Ir76b-GAL80, and corresponding controls. Arrows indicate presence/loss of GFP expression in the SEZ. (D) and (E) Expression pattern of experimental flies in the brain as visualized using UAS-CD8::GFP in green, with nc82 synaptic staining in gray. Note absence of cell bodies in the Ir25a-GAL4 brain. A, antenna; VCSO, ventral cibarial sense organ; LSO, labral sense organ; L, labellum; AL, antennal lobes; SEZ, subesophageal zone. In this and following figures, empty and filled black circles represent absence and presence of the indicated elements, respectively. In (A), boxes represent median with upper/lower quartiles. In (B), (D) and (E), circles represent yeast preference in single assays, with line representing the median and whiskers the interquartile range. ***p

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