Regulatory co-factors of XPO1 are recruited into VROs during TBSV replication.

Confocal microscopy images show the recruitment of eGFP-RanGAP1 (A), eGFP-RanGAP2 (B), or eGFP-RanBP1-1b (C) into VROs during TBSV replication. The bottom images in panels A, B and C show the subcellular localizations of eGFP-RanGAP1, eGFP-RanGAP2, or eGFP-RanBP1-1b in mock inoculated control plant cells. Yellow arrows indicate the nucleus, while VROs are marked with white arrows. Note these images were taken using H2B-CFP transgenic N. benthamiana marking the nucleus. Confocal microscopy images also show the associations of eGFP-RanGAP1 (D), eGFP-RanGAP2 (E), eGFP-RanBP1-1b (F) and p33-BFP replication protein with actin filament networks during TBSV replication. Scale bars represent 10 μm. (PDF)