To study the pharmacokinetics of cyclosporine A in Hong Kong Chinese stable renal transplant patients by a rapid and simple liquid chromatography tandem mass spectrometry.

Law Wai Keung. ; Thesis (M.Sc.)--Chinese University of Hong Kong, 2002. ; Includes bibliographical references (leaves 98-108). ; Abstracts in English and Chinese. ; Abstract --- p.v ; 摘要 --- p.viii ; Acknowledgement --- p.x ; List of Abbreviations --- p.i ; Index of tables --- p.xiv ; Index of figures --- p.xv ; Chapter 1. --- Introduction --- p.1 ; Chapter 2. --- Literature review --- p.3 ; Chapter 2.1 --- Immunosuppression in Organ Transplantation --- p.3 ; Chapter 2.2 --- Mechanism of Graft Rejection --- p.4 ; Chapter 2.3 --- Conventional immunosuppressive drugs --- p.4 ; Chapter 2.3.1 --- Corticosteriod --- p.6 ; Chapter 2.3.2 --- Azathioprine --- p.6 ; Chapter 2.3.3 --- Polyclonal antilymphocyte globulin and OKT3 --- p.7 ; Chapter 2.4 --- Cyclosporine A (CsA) --- p.8 ; Chapter 2.4.1 --- Mechanisms of CsA --- p.8 ; Chapter 2.4.2 --- Pharmacokinetics of CsA --- p.10 ; Chapter 2.4.2.1 --- Absorption --- p.10 ; Chapter 2.4.2.2 --- Distribution --- p.11 ; Chapter 2.4.2.3 --- Metabolism and elimination --- p.11 ; Chapter 2.4.2.4 --- Toxicity --- p.12 ; Chapter 2.4.3 --- Therapeutic drug monitoring of CsA --- p.13 ; Chapter 2.4.3.1 --- CsA trough monitoring --- p.13 ; Chapter 2.4.3.2 --- Full AUC monitoring --- p.15 ; Chapter 2.4.3.3 --- Limited sampling strategy --- p.16 ; Chapter 2.4.3.4 --- Two-hour post dose CsA level monitoring --- p.20 ; Chapter 2.4.4 --- Conventional techniques of measuring cyclosporine concentration --- p.23 ; Chapter 2.4.4.1 --- High performance liquid chromatography --- p.23 ; Chapter 2.4.4.2 --- Non-specific immunoassays --- p.25 ; Chapter 2.4.4.3 --- Specific radioimmunoassays --- p.26 ; Chapter 2.4.4.4 --- Specific fluorescent polarization immunoassay --- p.26 ; Chapter 2.4.4.5 --- Enzyme multiplied immunoassay technique --- p.28 ; Chapter 2.4.4.6 --- Cloned enzyme donor immunoassay --- p.29 ; Chapter 2.4.4.7 --- Summary for conventional techniques --- p.29 ; Chapter 2.5 --- Liquid chromatography mass spectrometry for CsA measurement --- p.30 ; Chapter 2.5.1 --- Main components of MS ...