Contributors: Leyva-Jiménez,FJ; Lozano-Sánchez,J; Arráez-Román,D; Segura-Carretero,A Research and Development Functional Food Centre, Health Science Technological Park, Granada, Spain. Ruiz-Malagón,AJ; Molina-Tijeras,JA; Diez-Echave,P; Vezza,T; Hidalgo-García,L; Rodríguez-Nogales,A; Gálvez,J CIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, Granada, Spain. Ruiz-Malagón,AJ; Gálvez,J Instituto de Investigación Biosanitaria de Granada (Ibs. GRANADA), Granada, Spain. Lozano-Sánchez,J Department of Nutrition and Food Science, Center for Biomedical Research (CIBM), University of Granada, Granada, Spain. Arráez-Román,D; Segura-Carretero,A Department of Analytical Chemistry, University of Granada, Granada, Spain. Cenis,JL; Lozano-Pérez,AA Departamento de Biotecnología, Genómica y Mejora Vegetal, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario, La Alberca (Murcia), Spain. Rodríguez-Nogales,A Servicio de Digestivo, Hospital Universitario Virgen de las Nieves, Granada, Spain.; This research was funded by the Junta de Andalucía (CTS 164) and by the Spanish Ministry of Economy and Competitiveness (AGL2015-67995-C3-3-R), with funds from the European Commission (FEDER/ERDF). Lozano-Pérez’s research contract is partially supported (80%) by the ERDF/FEDER Operational Programme of the Region of Murcia CCI Nº 2007ES161PO001 (Project No. 14-20/20). The CIBER-EHD is funded by the Instituto de Salud Carlos III. A.J. Ruiz-Malagón and L. Hidalgo-García are predoctoral fellows from University of Granada of “Programa de Doctorado: Medicina Clínica y Salud Pública” (Formación de Profesorado Universitario Program); J.A. Molina-Tijeras is a predoctoral fellow from University of Granada of “Programa de Doctorado: Nutrición y Ciencias de los Alimentos”. P. Diez-Echave is a predoctoral fellow from University of Granada of “Programa de Doctorado: Medicina Clínica y Salud Pública”, T. Vezza is a postdoctoral fellow from Instituto de Investigación Biosanitaria (Ibs.GRANADA) and A. Rodriguez-Nogales is postdoctoral fellow from Instituto de Salud Carlos III (Miguel Servet Program).
نبذة مختصرة : Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography-electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH· radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders. ; Yes
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