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Construction of a High‐Mannose‐Type Glycan Library by a Renewed Top‐Down Chemo‐Enzymatic Approach

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  • معلومة اضافية
    • بيانات النشر:
      Wiley, 2015.
    • الموضوع:
      2015
    • نبذة مختصرة :
      A comprehensive method for the construction of a high-mannose-type glycan library by systematic chemo-enzymatic trimming of a single Man9-based precursor was developed. It consists of the chemical synthesis of a non-natural tridecasaccharide precursor, the orthogonal demasking of the non-reducing ends, and trimming by glycosidases, which enabled a comprehensive synthesis of high-mannose-type glycans in their mono- or non-glucosylated forms. It employed glucose, isopropylidene, and N-acetylglucosamine groups for blocking the A-, B-, and C-arms, respectively. After systematic trimming of the precursor, thirty-seven high-mannose-type glycans were obtained. The power of the methodology was demonstrated by the enzymatic activity of human recombinant N-acetylglucosaminyltransferase-I toward M7-M3 glycans, clarifying the substrate specificity in the context of high-mannose-type glycans.
    • ISSN:
      1521-3765
      0947-6539
    • الرقم المعرف:
      10.1002/chem.201405781
    • Rights:
      CLOSED
    • الرقم المعرف:
      edsair.doi.dedup.....93ea318af98657c559120c1d3094d448