نبذة مختصرة : OBJECTIVESTo analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC‐RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA on the progression of CC‐RCC.PATIENTS AND METHODSWe quantified PSCA mRNA level in human RCC cell lines (ACHN, A704, KPK‐1, Caki‐1, and Caki‐2) and in 154 surgical tissue samples (81 from CC‐RCC, 73 from normal kidney) using real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR). The findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using confocal laser scanning light‐microscopy.RESULTSPSCA was overexpressed in all RCC cell lines. PSCA mRNA levels were significantly higher in CC‐RCC than in normal kidney tissue samples (P P = 0.028), and in advanced disease (T3‐T4) than in organ‐confined (T1‐T2) tumours (P = 0.016). There was significantly higher PSCA mRNA expression in patients with M1 than in those with M0 disease (P = 0.029). Patients in whom the lesions had high PSCA expression levels had a significantly worse prognosis than those with low PSCA expression levels (P = 0.044). Using immunohistochemical analysis there was markedly greater PSCA expression in CC‐RCC than in normal kidney, and in advanced‐disease high‐grade tumours than in organ‐confined low‐grade tumours.CONCLUSIONSA significant correlation was detected in the gene expression level of PSCA with histological grade, clinicopathological stage and prognosis in CC‐RCC. Our data indicate that PSCA is associated with carcinogenesis and progression of CC‐RCC.
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