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Development of a nano antibiotic paste for lesion sterilization and tissue repair technique in pediatric dentistry.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • الموضوع:
      Anti-Bacterial Agents*/pharmacology ; Anti-Bacterial Agents*/chemistry ; Anti-Bacterial Agents*/administration & dosage ; Metal Nanoparticles*/chemistry ; Nanoparticles*/chemistry; Microbial Sensitivity Tests ; Enterococcus faecalis/drug effects ; Humans ; Staphylococcus aureus/drug effects ; Escherichia coli/drug effects ; Silver/chemistry ; Silver/pharmacology ; Zinc Oxide/chemistry ; Zinc Oxide/pharmacology ; Streptococcus mutans/drug effects ; Chloramphenicol/pharmacology ; Chloramphenicol/chemistry ; Tetracycline/pharmacology ; Tetracycline/chemistry ; Ointments
    • نبذة مختصرة :
      This is an in vitro study aimed to develop a nano-antibiotic paste containing chloramphenicol, tetracycline, and silver and zinc oxide nanoparticles, evaluating its microbiological efficacy against four bacterial strains, determining its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against a commercial CTZ paste. Silver and zinc oxide nanoparticles (1 × 10 - 2 M) were synthesized and characterized by UV-Vis and DLS. A paste of tetracycline and chloramphenicol was prepared to a known concentration (10%), adding the synthesized nanoparticles and reducing the antibiotic percentage from the original proportions (- 4%). The commercial CTZ paste was then prepared following the manufacturer's instructions (13% concentration). Microdilution method and Kirby-Bauer assessment were used at three different concentrations to evaluate antimicrobial activity for both pastes against Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, and Escherichia coli. MBC for the nano antibiotic paste (10%) and commercial CTZ paste (13%) were 4.8 µg/ml and 12 µg/ml for S. aureus, 19.5 µg/ml and 12.8 µg/ml for E. coli, 19.5 µg/ml and 12.8 µg/ml for E. faecalis, 39 µg/ml and 25 µg/ml for S. mutans, respectively. For Kirby Bauer statistical difference was shown, favoring nano paste for C1 E. coli (p = 0.000262) and C2 E. coli (p = 0.000388), and for C3 S. mutans (p = 0.00749), and commercial CTZ paste, for C2 S. aureus (p = 0.0146) and C2 E. faecalis (p = 0.00033). Microbiological assays revealed that the commercial CTZ de Mexico™ paste (13%) exhibited superior bactericidal activity, with lower MBCs, than the Nano formulation (10%) containing 4% less antibiotic. Results offer in vitro doses for the strains.
      (© 2025. The Author(s).)
    • نبذة مختصرة :
      Declarations. Competing interests: The authors declare no competing interests.
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    • Contributed Indexing:
      Keywords: Anti-bacterial agents; Dental pulp necrosis; Nanoparticles; Tooth deciduous
    • الرقم المعرف:
      0 (Anti-Bacterial Agents)
      3M4G523W1G (Silver)
      SOI2LOH54Z (Zinc Oxide)
      66974FR9Q1 (Chloramphenicol)
      F8VB5M810T (Tetracycline)
      0 (Ointments)
    • الموضوع:
      Date Created: 20251230 Date Completed: 20251230 Latest Revision: 20260102
    • الموضوع:
      20260102
    • الرقم المعرف:
      PMC12749045
    • الرقم المعرف:
      10.1038/s41598-025-28545-x
    • الرقم المعرف:
      41462056