Characterization of the secondary structure, renaturation and physical ageing of gelatine adhesives.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Gelatin*/chemistry ; Adhesives*/chemistry; Animals ; Rheology ; Calorimetry, Differential Scanning ; Thermogravimetry ; Protein Structure, Secondary ; Spectroscopy, Fourier Transform Infrared ; X-Ray Diffraction

Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
Animal glues have been used since antiquity, but their popularity decreased in the twentieth century with the rise of synthetic adhesives. Currently they are primarily used in restoration of works of art. This study focuses on animal glue samples derived from bone and hide tissues used mainly for veiling and carpentry applications, examining their secondary structure, thermal and rheological properties, to shed light into their adhesive behaviour. Thermogravimetric analysis and differential scanning calorimetry highlight differences between hide and bone glues, showing that the latter are more hydrolysed. The calorimetric curves show varying values of denaturation enthalpy thus indicating a varying degree of gelatine renaturation. Additionally, the calorimetric analysis demonstrates the physical ageing of the glue samples, which is known to play a key role in maintaining their adhesive properties under specific storage conditions. The rheological data provide fundamental information on properties which are relevant for the use of animal glues as adhesives in various applications. XRD data suggest a low structural order in the samples investigated, where the amorphous component complicates the univocal interpretation of the spectra. FTIR reveal a relatively high content of β-structures in all commercial glue samples, as well as gelatine and collagen standards. Moreover, spectra of the glue films dried for 1 week show that the β-structures are recovered following dissolution in water and application. Therefore, β-structures seem crucial to explain the structure-dependent mechanical properties observed.
(© 2025. The Author(s).)

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P2022HWL7L MUR project PRIN 2022 PNRR ArtDECOW

Keywords: Animal glue; Gelatine; Gelatine adhesives; Hydrolysed collagen; Physical ageing; Secondary structure; Thermal analysis

9000-70-8 (Gelatin)
0 (Adhesives)

Date Created: 20250702 Date Completed: 20250702 Latest Revision: 20250704

20250704

PMC12216275

10.1038/s41598-025-04910-8

40595774