Uncovering seed vigor responsive miRNA in hybrid wheat and its parents by deep sequencing.

Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE

Original Publication: London : BioMed Central, [2000-

Triticum*/genetics ; Triticum*/growth & development ; MicroRNAs*/genetics ; MicroRNAs*/metabolism ; Seeds*/genetics ; Seeds*/growth & development ; High-Throughput Nucleotide Sequencing* ; Hybrid Vigor*/genetics; Gene Expression Regulation, Plant

Background: Two-line hybrid wheat technology system is one way to harness wheat heterosis both domestically and internationally. Seed vigor is a crucial parameter for assessing seed quality, as enhanced seed vigor can lead to yield increments of over 20% to a certain extent. MicroRNAs (miRNAs) were known to participate in the development and vigor of seed in plants, but its impact on seed vigor in two-line hybrid wheat remains poorly elucidated.
Results: The hybrid (BS1453/11GF5135) wheat exhibited superiority in seed vigor and anti-aging capacity, compared to its male parent (11GF5135, MP) and female parent (BS1453, FP). We identified four miRNAs associated with seed vigor, all of which are novel miRNAs. The majority of targets of miRNAs were related to ubiquitin ligases, kinases, sucrose synthases and hydrolases, involving in starch and sucrose metabolism, hydrolysis, catalysis, plant hormone signal transduction, and other pathways, which played crucial roles in seed development. Additionally, we also found miR531 was differentially expressed in both male parent and hybrid, and its target gene was a component of the E1 subunit of α-ketoate dehydrogenase complex, which interacted with dihydrolipoamide acetyltransferase (E2) and dihydrolipoyl dehydrogenase (E3). Finally, We established a presumptive interaction model to speculate the relationship of miR531 and seed vigor.
Conclusions: This study analyzed the seed vigor of two-line hybrid wheat, and screened seed vigor-related miRNAs. Meanwhile speculated the genetic relationship of hybrid and parents, in terms of miRNAs. Consequently, the present study provides new insights into the miRNA-mediated gene and protein interaction network that regulates seed vigor. These findings hold significance for enhancing the yield and quality of two-line hybrid wheat, facilitating its future applications.
(© 2024. The Author(s).)

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KJCX20210439 Science and Technology Innovation Project of BAAFS; KJCX20230415 Science and Technology Innovation Project of BAAFS; 2023ZD040230309 Sci-Tech Innovation 2030 Agenda

Keywords: MiRNA; RNA-seq; Seed vigor; Two-line hybrid wheat

0 (MicroRNAs)

Date Created: 20241022 Date Completed: 20241023 Latest Revision: 20241030

20250114

PMC11515737

10.1186/s12864-024-10878-y

39438825