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An Integrated Strategy for Establishing the Chemical Profile of Premna Microphylla Turcz. Leaves and Metabolites in Vivo.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • المصدر:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9215446 Publication Model: Print Cited Medium: Internet ISSN: 1944-7922 (Electronic) Linking ISSN: 10603271 NLM ISO Abbreviation: J AOAC Int Subsets: MEDLINE
    • بيانات النشر:
      Publication: 2020- : Oxford : Oxford University Press
      Original Publication: Arlington, VA : AOAC International, c1992-
    • الموضوع:
      Plant Leaves*/chemistry ; Plant Leaves*/metabolism ; Phytochemicals*/analysis ; Phytochemicals*/metabolism ; Plant Extracts*/chemistry; Chromatography, High Pressure Liquid/methods ; Tandem Mass Spectrometry/methods ; Flavonoids/analysis ; Terpenes/analysis ; Animals ; Mice ; Male
    • نبذة مختصرة :
      Background: Premna microphylla Turcz. (PMT) is a traditional food and medicinal plant, which has been used to treat cure hemostasis, rheumatism, and dysentery. However, there is still a lack of a clear understanding of the chemical profile of PMT and its metabolites in vivo.
      Objective: To establish a rapid and efficient analytical method for the identification of phytochemicals in PMT and their metabolites in vivo.
      Methods: First, the fingerprint of PMT was established by HPLC with method validation. Then, the phytochemical composition of PMT leaves was identified using ultra-performance liquid chromatography-quadrupole time-of-flight-tandem mass spectrometry (UPLC-QTOF-MS/MS). Finally, the prototype and correlated metabolites were detected after oral administration in mice to understand the absorption and metabolism of phytochemicals in vivo.
      Results: The results showed that the established HPLC method for fingerprint evaluation of PMT has good precision, repeatability, and stability. Additionally, a total of 103 phytochemicals were identified in PMT, including mainly flavonoids and terpenoids. Then, 37 prototype components and 20 derived metabolites in vivo were detected.
      Conclusion: In this study, we constructed a fingerprint method, which has good stability, precision, and repeatability, and the fingerprint of PMT was established. Then, the chemical profile of PMT in vitro and in vivo was determined. The results showed that flavonoids and terpenoids were the main phytochemicals in PMT, and methylation, sulfonation, and dihydroxylation were the main metabolic pathway in vivo.
      Highlights: The present study provides the phytochemical basis for subsequent study of pharmacological activity.
      (© The Author(s) 2024. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
    • Grant Information:
      2023ZF081 Zhejiang Province Traditional Chinese Medicine Science and Technology; GZY-ZJ-SY-2303 Key Laboratory for Molecular Medicine and Chinese Medicine Preparations; Shared Instrumentation Core Facility; Hangzhou Institute of Medicine; Chinese Academy of Sciences
    • الرقم المعرف:
      0 (Phytochemicals)
      0 (Flavonoids)
      0 (Terpenes)
      0 (Plant Extracts)
    • الموضوع:
      Date Created: 20241014 Date Completed: 20250424 Latest Revision: 20250515
    • الموضوع:
      20260130
    • الرقم المعرف:
      10.1093/jaoacint/qsae079
    • الرقم المعرف:
      39401003