Influence of nitrate supplementation on motor unit activity during recovery following a sustained ischemic contraction in recreationally active young males.

Publisher: Steinkopff Country of Publication: Germany NLM ID: 100888704 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1436-6215 (Electronic) Linking ISSN: 14366207 NLM ISO Abbreviation: Eur J Nutr Subsets: MEDLINE

Original Publication: Darmstadt, Germany : Steinkopff, 1999-

Cross-Over Studies* ; Nitrates*/administration & dosage ; Nitrates*/pharmacology ; Nitrates*/blood ; Dietary Supplements* ; Muscle, Skeletal*/drug effects ; Muscle, Skeletal*/physiology ; Electromyography*/methods; Humans ; Male ; Double-Blind Method ; Adult ; Young Adult ; Muscle Contraction/drug effects ; Isometric Contraction/drug effects ; Isometric Contraction/physiology ; Fruit and Vegetable Juices ; Beta vulgaris/chemistry ; Ischemia/physiopathology ; Regional Blood Flow/drug effects

Purpose: Dietary nitrate (NO 3^- ) supplementation enhances muscle blood flow and metabolic efficiency in hypoxia, however, its efficacy on neuromuscular function and specifically, the effect on motor unit (MU) activity is less clear. We investigated whether NO 3^- supplementation affected MU activity following a 3 min sustained ischemic contraction and whether this is influenced by blood flow restriction (BFR) during the recovery period.
Method: In a randomized, double-blinded, cross-over design, 14 males (mean ± SD, 25 ± 6 years) completed two trials following 5 days of supplementation with NO 3^- -rich (NIT) or NO 3^- -depleted (PLA) beetroot juice to modify plasma nitrite (NO 2^- ) concentration (482 ± 92 vs. 198 ± 48 nmol·L ^-1 , p < 0.001). Intramuscular electromyography was used to assess MU potential (MUP) size (duration and area) and mean firing rates (MUFR) during a 3 min submaximal (25% MVC) isometric contraction with BFR. These variables were also assessed during a 90 s recovery period with the first half completed with, and the second half completed without, BFR.
Results: The change in MUP area and MUFR, did not differ between conditions (all p > 0.05), but NIT elicited a reduction in MUP recovery time during brief isometric contractions (p < 0.001), and during recoveries with (p = 0.002) and without (p = 0.012) BFR.
Conclusion: These novel observations improve understanding of the effects of NO 3^- on the recovery of neuromuscular function post-exercise and might have implications for recovery of muscle contractile function.
Trial Registration: The study was registered on clinicaltrials.gov with ID of NCT05993715 on August 08, 2023.
(© 2024. The Author(s).)

Muscle Nerve. 2003 Aug;28(2):204-11. (PMID: 12872325)
J Appl Physiol (1985). 2014 Dec 15;117(12):1460-70. (PMID: 25301896)
Nat Rev Drug Discov. 2008 Feb;7(2):156-67. (PMID: 18167491)
Clin Exp Pharmacol Physiol. 2007 Apr;34(4):318-26. (PMID: 17324144)
Muscle Nerve. 2001 Jul;24(7):867-82. (PMID: 11410914)
J Am Coll Nutr. 2016;35(2):100-7. (PMID: 26885762)
Clin Neurophysiol. 2021 May;132(5):1089-1104. (PMID: 33774377)
Nutrients. 2022 Sep 03;14(17):. (PMID: 36079902)
J Appl Physiol (1985). 2005 Jul;99(1):197-203. (PMID: 15761090)
Front Physiol. 2019 Apr 26;10:449. (PMID: 31080415)
Front Physiol. 2019 May 10;10:545. (PMID: 31133877)
Physiol Rep. 2018 Jan;6(2):. (PMID: 29380952)
Biogerontology. 2016 Jun;17(3):485-96. (PMID: 26667009)
J Electromyogr Kinesiol. 2008 Feb;18(1):2-15. (PMID: 17113787)
Acta Physiol Scand Suppl. 1986;556:137-48. (PMID: 3471051)
Int J Sports Physiol Perform. 2019 May 21;14(6):706-710. (PMID: 30427246)
Annu Rev Nutr. 2018 Aug 21;38:303-328. (PMID: 30130468)
Am J Physiol Cell Physiol. 2002 Jul;283(1):C169-77. (PMID: 12055085)
Front Neurol. 2017 Apr 03;8:120. (PMID: 28421032)
Eur J Appl Physiol. 2016 Feb;116(2):353-62. (PMID: 26537365)
Circ Res. 2001 Feb 2;88(2):145-51. (PMID: 11157665)
Am J Physiol. 1997 Feb;272(2 Pt 1):C550-9. (PMID: 9124298)
J Physiol. 2009 Sep 1;587(Pt 17):4329-38. (PMID: 19596896)
J Physiol. 2011 Nov 15;589(Pt 22):5517-28. (PMID: 21911616)
Respir Physiol Neurobiol. 2012 Jul 1;182(2-3):53-9. (PMID: 22588047)
Eur J Appl Physiol. 2022 Jul;122(7):1683-1693. (PMID: 35460359)
Med Sci Sports Exerc. 2018 Oct;50(10):2122-2131. (PMID: 29727405)
J Appl Physiol (1985). 2013 Aug 1;115(3):325-36. (PMID: 23640589)
Sports Med. 2022 Oct;52(10):2537-2558. (PMID: 35604567)
J Neurophysiol. 2015 May 1;113(9):3186-96. (PMID: 25761952)
J Appl Physiol (1985). 2002 Apr;92(4):1585-93. (PMID: 11896025)
Exp Physiol. 2019 Jul;104(7):1100-1114. (PMID: 31004378)
IEEE Trans Neural Syst Rehabil Eng. 2004 Jun;12(2):166-76. (PMID: 15218931)
Eur J Sport Sci. 2015;15(8):712-9. (PMID: 26681629)
J Appl Physiol (1985). 2018 Oct 1;125(4):1218-1226. (PMID: 30024336)
Muscle Nerve. 1999 Feb;22(2):218-29. (PMID: 10024135)
Neurosci Lett. 1997 Dec 12;239(1):25-8. (PMID: 9547163)
Am J Physiol Cell Physiol. 2003 Dec;285(6):C1464-74. (PMID: 12917105)
Acta Physiol (Oxf). 2012 Dec;206(4):242-50. (PMID: 22762286)
Clin Nutr. 2018 Dec;37(6 Pt A):1852-1861. (PMID: 29108664)
Nutrients. 2016 Aug 18;8(8):. (PMID: 27548212)
J Physiol. 1986 Oct;379:451-9. (PMID: 3560001)
Med Sci Sports Exerc. 2017 Oct;49(10):2016-2024. (PMID: 28509762)
Front Physiol. 2019 Apr 24;10:404. (PMID: 31068827)
J Neurosci. 2013 Apr 24;33(17):7308-7324. (PMID: 23616539)
J Appl Physiol (1985). 1999 Apr;86(4):1337-46. (PMID: 10194220)
Wiley Interdiscip Rev Syst Biol Med. 2012 Sep-Oct;4(5):457-73. (PMID: 22811254)
Exerc Sport Sci Rev. 2018 Oct;46(4):240-246. (PMID: 30001270)
J Biol Chem. 2013 Sep 13;288(37):26473-9. (PMID: 23861393)
J Appl Physiol (1985). 1992 May;72(5):1631-48. (PMID: 1601767)
Exp Physiol. 2021 Jan;106(1):200-211. (PMID: 31912952)
Med Sci Sports Exerc. 2014 Dec;46(12):2234-43. (PMID: 24681572)
J Appl Physiol (1985). 2011 Mar;110(3):591-600. (PMID: 21071588)
Eur J Appl Physiol. 2000 Oct;83(2-3):190-9. (PMID: 11104060)
J Phys Ther Sci. 2014 Apr;26(4):621-4. (PMID: 24764647)
PLoS Biol. 2018 Apr 9;16(4):e2003611. (PMID: 29630591)
Eur J Appl Physiol. 2013 Jul;113(7):1673-84. (PMID: 23370859)
Eur J Neurosci. 2013 Jan;37(2):181-9. (PMID: 23121214)

Keywords: Beetroot juice; Electromyography; Motor unit; Nitric oxide; Recovery

ClinicalTrials.gov NCT05993715

0 (Nitrates)

Date Created: 20240529 Date Completed: 20240905 Latest Revision: 20240924

20250114

PMC11377467

10.1007/s00394-024-03440-9

38809323