Chromatography affinity resin with photosynthetically-sourced protein A ligand.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Staphylococcal Protein A*/chemistry ; Chromatography*; Plants, Genetically Modified/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Antibodies, Monoclonal/metabolism ; Immunoglobulin G/metabolism ; Plant Proteins/metabolism ; Chromatography, Affinity/methods ; Ligands

Green, photosynthesizing plants can be proficiently used as cost-effective, single-use, fully biodegradable bioreactors for environmentally-friendly production of a variety of valuable recombinant proteins. Being near-infinitely scalable and most energy-efficient in generating biomass, plants represent profoundly valid alternatives to conventionally used stationary fermenters. To validate this, we produced a plastome-engineered tobacco bioreactor line expressing a recombinant variant of the protein A from Staphylococcus aureus, an affinity ligand widely useful in antibody purification processes, reaching accumulation levels up to ~ 250 mg per 1 kg of fresh leaf biomass. Chromatography resin manufactured from photosynthetically-sourced recombinant protein A ligand conjugated to agarose beads demonstrated the innate pH-driven ability to bind and elute IgG-type antibodies and allowed one-step efficient purification of functional monoclonal antibodies from the supernatants of the producing hybridomas. The results of this study emphasize the versatility of plant-based recombinant protein production and illustrate its vast potential in reducing the cost of diverse biotechnological applications, particularly the downstream processing and purification of monoclonal antibodies.
(© 2024. The Author(s).)

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CCIP 529379-18 Natural Sciences and Engineering Research Council of Canada

0 (Staphylococcal Protein A)
0 (Ligands)
0 (Recombinant Proteins)
0 (Antibodies, Monoclonal)
0 (Immunoglobulin G)
0 (Plant Proteins)

Date Created: 20240415 Date Completed: 20240417 Latest Revision: 20240425

20260130

PMC11018848

10.1038/s41598-024-59266-2

38622266