Adenine-induced chronic kidney disease induces a similar skeletal phenotype in male and female C57BL/6 mice with more severe deficits in cortical bone properties of male mice.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Adenine/*pharmacology ; Bone Diseases, Metabolic/*genetics ; Parathyroid Hormone/*blood ; Renal Insufficiency, Chronic/*genetics; Adenine/adverse effects ; Animals ; Bone Diseases, Metabolic/blood ; Bone Diseases, Metabolic/chemically induced ; Bone Diseases, Metabolic/pathology ; Bone and Bones/metabolism ; Bone and Bones/pathology ; Cortical Bone/metabolism ; Cortical Bone/pathology ; Diet ; Disease Models, Animal ; Female ; Femur/drug effects ; Femur/metabolism ; Femur/pathology ; Humans ; Kidney/metabolism ; Kidney/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Muscle, Skeletal/metabolism ; Muscle, Skeletal/pathology ; Phenotype ; Porosity/drug effects ; Renal Insufficiency, Chronic/blood ; Renal Insufficiency, Chronic/chemically induced ; Renal Insufficiency, Chronic/pathology ; Sex Characteristics

Competing Interests: The authors have declared that no competing interest exist.
Chronic kidney disease (CKD) causes bone loss, particularly in cortical bone, through formation of cortical pores which lead to skeletal fragility. Animal models of CKD have shown variability in the skeletal response to CKD between males and females suggesting sex may play a role in this variation. Our aim was to compare the impact of adenine-induced CKD on cortical parameters in skeletally mature male and female C57Bl/6 mice. After 10-weeks of adenine-induced CKD, both male and female adenine mice had high serum parathyroid hormone (PTH), high bone turnover, and cortical porosity compared to non-CKD controls. Both sexes had lower cortical thickness, but only male mice had lower cortical bone area. CKD imparted greater deficits in mechanical properties of male mice compared to female mice. These data demonstrate that both male and female mice develop high PTH/high bone turnover in response to adenine-induced CKD and that cortical bone phenotypes are slightly more severe in males, particularly in mechanical properties deficits.

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F32 DK122731 United States DK NIDDK NIH HHS

0 (Parathyroid Hormone)
JAC85A2161 (Adenine)

Date Created: 20210423 Date Completed: 20210929 Latest Revision: 20210929

20250114

PMC8064570

10.1371/journal.pone.0250438

33891630