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Knockdown of long non-coding RNA LEF1-AS1 attenuates apoptosis and inflammatory injury of microglia cells following spinal cord injury.
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- معلومة اضافية
- المصدر:
Publisher: BioMed Central Country of Publication: England NLM ID: 101265112 Publication Model: Electronic Cited Medium: Internet ISSN: 1749-799X (Electronic) Linking ISSN: 1749799X NLM ISO Abbreviation: J Orthop Surg Res Subsets: MEDLINE
- بيانات النشر:
Original Publication: London : BioMed Central, 2006-
- الموضوع:
- نبذة مختصرة :
Background: Spinal cord injury (SCI) is associated with health burden both at personal and societal levels. Recent assessments on the role of lncRNAs in SCI regulation have matured. Therefore, to comprehensively explore the function of lncRNA LEF1-AS1 in SCI, there is an urgent need to understand its occurrence and development.
Methods: Using in vitro experiments, we used lipopolysaccharide (LPS) to treat and establish the SCI model primarily on microglial cells. Gain- and loss of function assays of LEF1-AS1 and miR-222-5p were conducted. Cell viability and apoptosis of microglial cells were assessed via CCK8 assay and flow cytometry, respectively. Adult Sprague-Dawley (SD) rats were randomly divided into four groups: Control, SCI, sh-NC, and sh-LEF-AS1 groups. ELISA test was used to determine the expression of TNF-α and IL-6, whereas the protein level of apoptotic-related markers (Bcl-2, Bax, and cleaved caspase-3) was assessed using Western blot technique.
Results: We revealed that LncRNA LEF1-AS1 was distinctly upregulated, whereas miR-222-5p was significantly downregulated in LPS-treated SCI and microglial cells. However, LEF1-AS1 knockdown enhanced cell viability, inhibited apoptosis, as well as inflammation of LPS-mediated microglial cells. On the contrary, miR-222-5p upregulation decreased cell viability, promoted apoptosis, and inflammation of microglial cells. Mechanistically, LEF1-AS1 served as a competitive endogenous RNA (ceRNA) by sponging miR-222-5p, targeting RAMP3. RAMP3 overexpression attenuated LEF1-AS1-mediated protective effects on LPS-mediated microglial cells from apoptosis and inflammation.
Conclusion: In summary, these findings ascertain that knockdown of LEF1-AS1 impedes SCI progression via the miR-222-5p/RAMP3 axis.
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- Grant Information:
MB2019001, WKZL2018004 Scientific Research Project of Nantong Health Commission
- Contributed Indexing:
Keywords: Apoptosis; Inflammation; LEF1-AS1; Spinal cord injury; ceRNA; miR-222-5p
- الرقم المعرف:
0 (Lef1 protein, rat)
0 (Lymphoid Enhancer-Binding Factor 1)
0 (MIRN222 microRNA, rat)
0 (MicroRNAs)
0 (RNA, Long Noncoding)
0 (Ramp3 protein, rat)
0 (Receptor Activity-Modifying Protein 3)
- الموضوع:
Date Created: 20210107 Date Completed: 20210712 Latest Revision: 20240226
- الموضوع:
20250114
- الرقم المعرف:
PMC7786481
- الرقم المعرف:
10.1186/s13018-020-02041-6
- الرقم المعرف:
33407665
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