Useful In Vitro Techniques to Evaluate the Mucoadhesive Properties of Hyaluronic Acid-Based Ocular Delivery Systems.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101534003 Publication Model: Electronic Cited Medium: Print ISSN: 1999-4923 (Print) Linking ISSN: 19994923 NLM ISO Abbreviation: Pharmaceutics Subsets: PubMed not MEDLINE

Original Publication: Basel, Switzerland : MDPI

Polymer-based eye drops are the most used drug delivery system to treat dry eye disease (DED). Therefore, the mucoadhesion between the polymer and the ocular mucin is crucial to ensure the efficacy of the treatment. In this context, the present study aimed to evaluate the potential use of in vitro methods to study the mucoadhesion of eye drop solutions and, specifically to evaluate the efficacy of two hyaluronic acid-based formulations (HA), HA 0.15% and 0.30% ( w / v ) to treat DED. Rheology methods and zeta potential determination were used to study the mucoadhesive properties of both eye drop solutions. All results indicated that interactions occurred between the mucin and the HA, being stronger with HA 0.30%, due to the physical entanglements and hydrogen bounding. In vitro tests on ARPE-19 cell line were performed using a 2D and a 3D dry eye model and the results have shown that pre-treated cells with HA showed a morphology more similar to the hydrated cells in both products, with a high survival rate. The in vitro techniques used in this study have been shown to be suitable to evaluate and predict mucoadhesive properties and the efficacy of the eye drops on relief or treatment of DED. The results obtained from these methods may help in inferring possible in vivo effects.

Biomacromolecules. 2014 Oct 13;15(10):3550-8. (PMID: 25122160)
Exp Eye Res. 2013 Dec;117:1-3. (PMID: 24280033)
Biomacromolecules. 2015 Mar 9;16(3):924-35. (PMID: 25630032)
Curr Opin Ophthalmol. 2011 Jul;22(4):279-82. (PMID: 21597374)
Adv Drug Deliv Rev. 2005 Nov 3;57(11):1595-639. (PMID: 16198021)
Clin Ophthalmol. 2015 Nov 02;9:2039-47. (PMID: 26586933)
Drug Dev Ind Pharm. 2016;42(4):514-24. (PMID: 26407208)
J Ophthalmic Vis Res. 2011 Jul;6(3):192-8. (PMID: 22454735)
F1000Res. 2015 Aug 25;4:622. (PMID: 26594344)
Carbohydr Polym. 2016 Nov 20;153:275-283. (PMID: 27561497)
Int J Pharm. 2004 Sep 10;282(1-2):115-30. (PMID: 15336387)
Semin Ophthalmol. 2010 May;25(3):84-93. (PMID: 20590418)
Colloids Surf B Biointerfaces. 2012 Apr 1;92:353-9. (PMID: 22209653)
Mar Drugs. 2017 Dec 01;15(12):null. (PMID: 29194378)
Exp Eye Res. 1996 Feb;62(2):155-69. (PMID: 8698076)
Exp Eye Res. 2004 Mar;78(3):347-60. (PMID: 15106912)
Acta Ophthalmol (Copenh). 1988 Oct;66(5):485-9. (PMID: 3218470)
Ocul Surf. 2010 Oct;8(4):185-92. (PMID: 20964981)
Exp Eye Res. 1973 May 10;15(5):515-25. (PMID: 4712544)
Biomacromolecules. 2009 Sep 14;10(9):2427-35. (PMID: 19642670)
Pharm Res. 1990 May;7(5):491-5. (PMID: 1694990)
J Pharm Pharmacol. 2001 Dec;53(12):1589-99. (PMID: 11804389)
Biochim Biophys Acta. 2016 Oct;1858(10):2421-2430. (PMID: 26898663)
J Ophthalmic Vis Res. 2009 Oct;4(4):238-52. (PMID: 23198080)
Cornea. 2012 Nov;31 Suppl 1:S32-6. (PMID: 23038032)
Surv Ophthalmol. 2007 Jul-Aug;52(4):369-74. (PMID: 17574063)
Int J Toxicol. 2009 Jul-Aug;28(4 Suppl):5-67. (PMID: 19636067)
Saudi J Ophthalmol. 2014 Jan;28(1):19-30. (PMID: 24526854)
Ocul Surf. 2007 Apr;5(2):75-92. (PMID: 17508116)

Keywords: dry eye disease; eye drops; hyaluronic acid; in vitro tests; medical devices; mucoadhesion

Date Created: 20180804 Latest Revision: 20201001

20250114

PMC6161121

10.3390/pharmaceutics10030110

30071584