Editing proteins inside a cell.

Protein precursors; Amino acid sequence; Genetic code; Nucleotide sequence; Amino group; Protein-protein interactions; Genetic translation

The article discusses a new method for editing proteins within living mammalian cells using split inteins, small protein segments that can undergo self-sustained protein splicing reactions. This technique allows for the insertion of various protein fragments, including noncanonical amino acids and fluorescence tags, into endogenous proteins. While this method shows promise for studying protein modifications in a cellular context, further development is needed to improve efficiency and expand its applications. The research by Beyer et al. and Hua et al. demonstrates the potential of protein transposition for studying time-dependent cellular processes and modifying large protein complexes without disrupting cellular functions. [Extracted from the article]

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