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Analysis of effect of moxibustion intervention on cytotoxic T-lymphocyte antigen-4 in immunosuppressed rabbits. (English)

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  • معلومة اضافية
    • نبذة مختصرة :
      Objective: To compare similarities and differences in expression changes of cytotoxic T-lymphocyte antigen-4 (CT-LA-4) and programmed death receptor 1 (PD-1) in immunosuppressed rabbits under different moxibustion interventions. Methods: Twenty large-eared white rabbits were randomly divided into normal group, model group, moxa stick moxibustion (MSM) group and herbal cake-partitioned moxibustion (HPM) group, with five rabbits in each group. CTX-induced immunosuppressed models were prepared by intraperitoneal injection for 7 consecutive days. After successful modeling, MSM and HPM were performed on alternate days for 10 treatments. Rabbits were anesthetized after treatment, and serum, liver and spleen were collected. Serum PD-1 and PD-L1 contents were detected by ELISA. PD-1 in liver tissue was detected by immunohistochemistry, and CTLA-4 mRNA in liver and spleen tissues were detected by RT-qPCR. Results: Both HPM and MSM could reduce PD-1 and PD-L1 levels due to immunosuppression, and could effectively suppress elevated levels of CTLA-4 in spleen and PD-1 and CTLA-4 in liver, which were statistically different compared with immunosuppression model group (P<0.05). Pearson correlation test showed that CTLA-4 and PD-1 in liver tissue had significant positive correlation (r=0.780 7, P<0.001). Conclusion: HPM can improve body immune function by regulating multiple immunosuppressive sites. [ABSTRACT FROM AUTHOR]
    • نبذة مختصرة :
      Copyright of Chinese Journal of Immunology is the property of Medical Periodical Society of Jilin and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)