The use of biosensors to study GPCR function: applications for high-content screening.

Publisher: Taylor & Francis Country of Publication: England NLM ID: 9315376 Publication Model: Print Cited Medium: Print ISSN: 1060-6823 (Print) Linking ISSN: 10606823 NLM ISO Abbreviation: Recept Channels Subsets: MEDLINE

Publication: London : Taylor & Francis
Original Publication: Yverdon, Switzerland : Harwood Academic Publishers : Distributed by STBS, c1993-

Biosensing Techniques*; Biochemistry/*methods ; Biotechnology/*methods ; GTP-Binding Proteins/*chemistry ; Receptors, Cell Surface/*chemistry; Animals ; Arrestins/chemistry ; Calcium/metabolism ; Cell Membrane/metabolism ; Cyclic AMP/metabolism ; Green Fluorescent Proteins ; Humans ; Ligands ; Luminescent Proteins/metabolism ; Models, Biological ; Protein Binding ; Transferrin/chemistry

Plasma membrane-associated G protein-coupled receptors (GPCRs) initiate the transmission of multiple intracellular signals leading to a myriad of physiological and pathophysiological effects. The downstream signaling events associated with occupation of the GPCR and activation of the G-protein include the generation of numerous second messenger molecules to provide the necessary signal amplification within the appropriate intracellular compartment to transmit a specific signal from the cell surface to the cell interior. The complex process of signal transmission also requires a series of highly orchestrated events which includes the translocation of cellular proteins to discreet intracellular destinations. A better understanding of these events has made it possible to design assays to examine multiple endpoints within whole cells. In this review we describe recent advances in assay biology and instrumentation useful for broadening our understanding of the molecular events associated with GPCR activation. This review will focus on novel cell-based approaches using fluorescent biosensors, such as fluorescent dyes and fluorescent protein tags, to generate information-rich data from multiple cellular targets--a process that has been referred to as high-content screening. High-content screening applications will be discussed as they pertain to specific signal transduction cascades initiated upon GPCR activation and examples of specific biosensors will be provided.

90

0 (Arrestins)
0 (Ligands)
0 (Luminescent Proteins)
0 (Receptors, Cell Surface)
0 (Transferrin)
147336-22-9 (Green Fluorescent Proteins)
E0399OZS9N (Cyclic AMP)
EC 3.6.1.- (GTP-Binding Proteins)
SY7Q814VUP (Calcium)

Date Created: 20030415 Date Completed: 20030625 Latest Revision: 20200825

20250114

12690960