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The Crystal Structure of Engineered Nitroreductase NTR 2.0 and Impact of F70A and F108Y Substitutions on Substrate Specificity.

• Authors : Sharrock, Abigail V.¹ (AUTHOR) ; Mumm, Jeff S.² (AUTHOR) ; Bagdžiūnas, Gintautas³ (AUTHOR)

Subjects: *CRYSTAL structure; *VIBRIO vulnificus; *BACTERIAL enzymes

• Source: International Journal of Molecular Sciences. Apr2023, Vol. 24 Issue 7, p6633. 16p.

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A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

• Authors : Yan, Kai Cheng; Gardiner, Jordan E.; Sedgwick, Adam C.

Subjects: /dk/atira/pure/subjectarea/asjc/2500/2504; name=Electronic; Optical and Magnetic Materials

• Source: Yan , K C , Gardiner , J E , Sedgwick , A C , Thet , N , Heylen , R A , James , T D , Jenkins , A T A & He , X P 2023 , ' A TCF-based fluorescent probe to determine

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The Crystal Structure of Engineered Nitroreductase NTR 2.0 and Impact of F70A and F108Y Substitutions on Substrate Specificity

• Authors : Abigail V. Sharrock; Jeff S. Mumm; Gintautas Bagdžiūnas

Subjects: type I nitroreductase; NfsB; crystal structureagris

• Source: International Journal of Molecular Sciences; Volume 24; Issue 7; Pages: 6633

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CytoCy5S™, a compound of many structures. in vitro and in vivo evaluation of four near-infrared fluorescent substrates of nitroreductase (NTR)

• Authors : García de Jalón, Elvira; Ruiz de Garibay, Gorka; Haug, Bengt Erik

• Source: Dyes and Pigments ; volume 196, page 109553 ; ISSN 0143-7208

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A zebrafish screen reveals Renin-angiotensin system inhibitors as neuroprotective via mitochondrial restoration in dopamine neurons.

• Authors : Kim, Gha-Hyun J; Mo, Han; Liu, Harrison

Subjects: D. melanogaster; electronic health records; genetics

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A zebrafish screen reveals Renin-angiotensin system inhibitors as neuroprotective via mitochondrial restoration in dopamine neurons

• Authors : Gha-Hyun J Kim; Han Mo; Harrison Liu

Subjects: phenotypic screening; glucocerebrosidase (GBA); parkin, pink1, a-synuclein, dj-1

• Source: eLife, Vol 10 (2021)

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Engineering the Escherichia coli Nitroreductase NfsA to Create a Flexible Enzyme-Prodrug Activation System

• Authors : Abigail V. Sharrock; Sarah P. McManaway; Michelle H. Rich

Subjects: nitroreductase (NTR); bystander effect; targeted cell ablation

• Source: Frontiers in Pharmacology, Vol 12 (2021)

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Figure 7—figure supplement 3. Genetic ablation of neurons decreases pancreatic endocrine cell numbers. ; (A) Genetic ablation in Tg(elavl3:Gal4-VP16); Tg(UAS:NTR-mCherry) zebrafish expressing nitroreductase (NTR) in post-mitotic neurons. Treatment with 2 mM metronidazole (MTZ) from 24 to 80 hpf triggers cell death in NTR expressing cells. Whole mount immunostaining at 80 hpf for Insulin (beta cells), Somatostatin (delta cells), and DAPI (DNA). (B–D) Quantification of primary islet mass (B), beta cell number (C), and delta cell number (D), mean ± SEM, n = 9–11 animals, p-values from t tests are presented.

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Establishment of a Drug Screening Model for Cardiac Complications of Acute Renal Failure

• Authors : Shuyi Liao; Wenmin Yang; Ting Yu

Subjects: acute renal failure (ARF); cardiorenal syndrome (CRS); zebrafish modelagris

• Source: Biomolecules; Volume 11; Issue 9; Pages: 1370

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Figure 1. Physical proximity between the end-feet of spinal cord radial glia and extraneural vessels during development. ; (A) Lateral view of a 154 hpf Tg(kdrl:ras-mcherry);TgBAC(gfap:gfap-EGFP) trunk. The region inside the dashed line corresponds to the 10 somites where all analyses and quantifications were performed. Scale bar, 200 µm. (B–B”) High magnification images of a Tg(kdrl:ras-mcherry);TgBAC(gfap:gfap-EGFP) trunk at 154 hpf. End-feet of radial glia and ISVs or VTAs are physically adjacent. ISVs: intersegmental vessels, VTAs: vertebral arteries. Scale bar, 100 µm. (C–C’) High magnification confocal single-plane images of 154 hpf Tg(kdrl:ras-mcherry);TgBAC(gfap:gfap-EGFP) trunk section counterstained with DAPI. Radial glia end-feet lie in close proximity to ISVs (C’). Scale bars, 20 µm. (D–D”) 60 hpf Tg(gfap:EGFP);TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR) trunk spinal cord section. EGFP+ radial glia and mCherry+ cells are largely co-localized. Scale bar, 20 µm. (E–E”) 60 hpf TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR) trunk spinal cord section immunostained for HuC/HuD (green). EGFP+ neurons and mCherry+ cells are largely segregated. Scale bar, 20 µm. (F) Time course of nitroreductase (NTR)/metronidazole (Mtz)-mediated cell ablation of radial glia for the panels (G–I”). (G) 30 hpf TgBAC(gfap:gfap-EGFP);TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR) trunk. TgBAC(gfap:Gfap-EGFP) expression and TgBAC(gfap:gal4ff);Tg(UAS:mCherry-NTR) expression are observed in the spinal cord in an overlapping manner. SC: spinal cord. Scale bar, 100 µm. (H–H” and I–I”) 82 hpf TgBAC(gfap:gfap-EGFP);TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR) trunk after treatment with DMSO (H–H”) or 2 mM Mtz (I–I”) starting at 30 hpf. Unlike DMSO-treated fish that show strong co-expression of TgBAC(gfap:Gfap-EGFP) and TgBAC(gfap:gal4ff);Tg(UAS:mCherry-NTR) in their spinal cord, Mtz-treated fish show a dramatic reduction of this co-expression. Scale bar, 100 µm.

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