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CRISPR/Cas9 Gene Targeting plus Nanopore DNA Sequencing with the Plasmid pBR322 in the Classroom

• Authors : Röbbe Wünschiers (ORCID 0000-0002-2824-4707); Robert Maximilian Leidenfrost (ORCID 0000-0003-0494-2612); Hauke Holtorf

Subjects: Genetics; Science Instruction; Teaching Methods

• Source: Journal of Microbiology & Biology Education. 2024 25(2).6

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Full-Length Transcriptome Profile of Apis cerana Revealed by Nanopore Sequencing.

• Authors : Hu XF; Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Jiangxi Agricultural University, Nanchang 330045, China.; Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China.

Subjects: Transcriptome*/Transcriptome*/Transcriptome*/genetics ; Nanopore Sequencing*/Nanopore Sequencing*/Nanopore Sequencing*/methods ; Alternative Splicing*

• Source: International journal of molecular sciences [Int J Mol Sci] 2024 Oct 09; Vol. 25 (19). Date of Electronic Publication: 2024 Oct 09.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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Nanopore sequencing with unique molecular identifiers enables accurate mutation analysis and haplotyping in the complex lipoprotein(a) KIV-2 VNTR.

• Authors : Amstler S; Institute of Genetic Epidemiology, Medical University of Innsbruck, Innsbruck, Austria.; Streiter G

Subjects: Haplotypes* ; Minisatellite Repeats* ; Lipoprotein(a)*/Lipoprotein(a)*/Lipoprotein(a)*/genetics

• Source: Genome medicine [Genome Med] 2024 Oct 08; Vol. 16 (1), pp. 117. Date of Electronic Publication: 2024 Oct 08.Publisher: BioMed Central Country of Publication: England NLM ID: 101475844 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-994X

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Prokaryotic communities profiling of Indonesian hot springs using long-read Oxford Nanopore sequencing.

• Authors : Wirajana IN; Department of Chemistry, Faculty of Mathematics and Natural Science, Udayana University, Jimbaran-Badung, Bali, 18036, Indonesia.; Ariantari NP

Subjects: Hot Springs*/Hot Springs*/Hot Springs*/microbiology ; RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/genetics ; Nanopore Sequencing*/Nanopore Sequencing*/Nanopore Sequencing*/methods

• Source: BMC research notes [BMC Res Notes] 2024 Oct 02; Vol. 17 (1), pp. 286. Date of Electronic Publication: 2024 Oct 02.Publisher: Biomed Central Country of Publication: England NLM ID: 101462768 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-0500

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Evaluation of Commercial RNA Extraction Protocols for Avian Influenza Virus Using Nanopore Metagenomic Sequencing.

• Authors : Chaves M; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.; Hashish A

Subjects: Influenza in Birds*/Influenza in Birds*/Influenza in Birds*/virology ; Influenza in Birds*/Influenza in Birds*/Influenza in Birds*/diagnosis ; RNA, Viral*/RNA, Viral*/RNA, Viral*/genetics

• Source: Viruses [Viruses] 2024 Sep 07; Vol. 16 (9). Date of Electronic Publication: 2024 Sep 07.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915

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Nanopore Deep Sequencing as a Tool to Characterize and Quantify Aberrant Splicing Caused by Variants in Inherited Retinal Dystrophy Genes.

• Authors : Maggi J; Institute of Medical Molecular Genetics, University of Zurich, 8952 Schlieren, Switzerland.; Feil S

Subjects: Retinal Dystrophies*/Retinal Dystrophies*/Retinal Dystrophies*/genetics ; Retinal Dystrophies*/Retinal Dystrophies*/Retinal Dystrophies*/diagnosis ; Nanopore Sequencing*/Nanopore Sequencing*/Nanopore Sequencing*/methods

• Source: International journal of molecular sciences [Int J Mol Sci] 2024 Sep 03; Vol. 25 (17). Date of Electronic Publication: 2024 Sep 03.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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Analysis of bacterial transcriptome and epitranscriptome using nanopore direct RNA sequencing.

• Authors : Tan L; Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, China.; Guo Z

Subjects: Escherichia coli*/Escherichia coli*/Escherichia coli*/genetics ; Escherichia coli*/Escherichia coli*/Escherichia coli*/metabolism ; Staphylococcus aureus*/Staphylococcus aureus*/Staphylococcus aureus*/genetics

• Source: Nucleic acids research [Nucleic Acids Res] 2024 Aug 27; Vol. 52 (15), pp. 8746-8762.Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN:

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A case of an Angelman-syndrome caused by an intragenic duplication of UBE3A uncovered by adaptive nanopore sequencing.

• Authors : Holthöfer L; Institute for Human Genetics, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.; Diederich S

Subjects: Angelman Syndrome*/Angelman Syndrome*/Angelman Syndrome*/genetics ; Angelman Syndrome*/Angelman Syndrome*/Angelman Syndrome*/diagnosis ; Ubiquitin-Protein Ligases*/Ubiquitin-Protein Ligases*/Ubiquitin-Protein Ligases*/genetics

• Source: Clinical epigenetics [Clin Epigenetics] 2024 Aug 02; Vol. 16 (1), pp. 101. Date of Electronic Publication: 2024 Aug 02.Publisher: BiomedCentral Country of Publication: Germany NLM ID: 101516977 Publication Model: Electronic Cited Medium: Internet ISSN: 1868-7083

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Integration of solid-state nanopore arrays via dry bonding to photostructured microfluidic networks

• Authors : Jones, Peter D.; Mierzejewski, Michael

Subjects: Condensed Matter - Mesoscale and Nanoscale Physics

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The Future of Decoding Non-Standard Nucleotides: Leveraging Nanopore Sequencing for Expanded Genetic Codes

• Authors : Shim, Hyunjin

Subjects: Quantitative Biology - Genomics; Quantitative Biology - Populations and Evolution

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