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Effects of maternal consumption of morphine on rat skeletal system development

• Authors : Maryam Saeidinezhad; Vahid Razban; Hosein Safizadeh

Subjects: Morphine dependency; Skeletal system development; Ossification center

• Source: BMC Musculoskeletal Disorders, Vol 22, Iss 1, Pp 1-10 (2021)

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Additional file 1 of Effects of maternal consumption of morphine on rat skeletal system development

• Authors : Maryam Saeidinezhad (10799777); Vahid Razban (4781013); Hosein Safizadeh (10799780)

Subjects: Biochemistry; Medicine; Microbiology

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Effects of maternal consumption of morphine on rat skeletal system development.

• Authors : Saeidinezhad M; Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Somayeh Cross-road, Sajad Boulevard, Ebnesina Street, Kerman, 7619813159, Iran.; Department of Anatomical Sciences, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

Subjects: Chondrogenesis* ; Morphine*/Morphine*/Morphine*/adverse effects; Animals

• Source: BMC musculoskeletal disorders [BMC Musculoskelet Disord] 2021 May 13; Vol. 22 (1), pp. 435. Date of Electronic Publication: 2021 May 13.Publisher: BioMed Central Country of Publication: England NLM ID: 100968565 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2474

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High-fat diet decreases energy expenditure and expression of genes controlling lipid metabolism, mitochondrial function and skeletal system development in the adipose tissue, along with increased expression of extracellular matrix remodelling- and inflammation-related genes

• Authors : Choi, Myung-Sook; Kim, Young-Je; Kwon, Eun-Young

• Source: British Journal of Nutrition ; volume 113, issue 6, page 867-877 ; ISSN 0007-1145 1475-2662

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Comment on Choi et al.: High-fat diet decreases energy expenditure and expression of genes controlling lipid metabolism, mitochondrial function and skeletal system development in the adipose tissue, along with increased expression of extracellular matrix remodelling- and inflammation-related genes

• Authors : Sobolewska, Aleksandra; Włodarczyk, Marcin; Włodarczyk, Krzysztof

• Source: British Journal of Nutrition ; volume 114, issue 4, page 497-498 ; ISSN 0007-1145 1475-2662

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Figure 1—figure supplement 1. After induction of differentiation, the hMSCs cells undergo a hyperproliferative phase within the first 2 days followed by growth arrest and differentiation. ; The dynamics of this transition can be revealed by: (A) Western blot for retinoblastoma2 (pRB2, also known as p130) and Cyclin E. Cyclin E is expressed in naïve proliferating (day0) and hyperproliferating (day2) hMSCs and pRB2 (p130) is abundant and hyper-phosphorylated (marked by*) when cyclin E is present (Howard et al., 2000). pRB2 becomes hypo-phosphorylated and reduced in amounts when cyclin E is downregulated and cells cease to proliferate and start to differentiate. The antibodies used for Western blotting are pRB2 (Santa Cruz, SC317) CyclinE (mab, HE12). (B) Microarray analyses done in three biological replicates in hMSCs at the indicated time points using Affymetrix gene chips (HT_HG-U133_Plus_PM). The data were analyzed by ‘array tools’ and represented as heatmaps. In the right hand side, the Pie chart represents the enrichment of key words from genes that were significantly induced (1.5 fold) following induction of osteogenic differentiation at day 2 (n = 537), day 5 (n = 590) or day 10 (n = 641) or reduced at day 10 (n = 578) compared to naïve hMSCs analysed by DAVID (Huang et al., 2009). The global gene expression profile showed genes related to ‘induction of cell proliferation’ as well as ‘skeletal system development’ at day 2. The ‘proliferation associated genes’ were downregulated at day 10. ‘Skeletal system developmental genes’ continued to increase in expression at later time points (day 5 and 10). (C) Alizarin red staining in naive or late time points of osteogenic differentiation (15 days and 21 days) shows strong red staining representing calcium precipitates after 21 days. The pictures were taken using Leica camera (EC3) attached to an inverted microscope at 20X. A scale bar (100 µm) is shown in the picture. Together, these results confirmed that the earliest time point (day 2) represents a hyper-proliferative, but committed state (pre-osteoblasts), whereas day 10 reflects the immature-osteoblasts stage). Mineralization and osteoblasts maturation starts to take place after 15 days of differentiation. (D) Pseudo-colored 2D histogram showing the H3K27me3 ChIP-seq signal quantified at gene transcripts from start to end ± 1 Kb (n = 44,451) in naive hMSCs (day 0) (Y-axis) and at day 10 of osteogenic differentiation (X-axis) in relation to changes in H3K4me3 levels. H3K27me3-positive genomic regions (n = 44,451) are pseudo-colored according to the average log2 fold changes in H3K4me3 (day10/naive hMSC) upon osteogenic differentiation. Regions that gain H3K4me3 are colored red, and regions with loss in H3K4me3 are colored blue. A bar showing the relationship between coloring and H3K4me3 changes can be seen at the side of the plot. (E) and (F) Average distribution of H3K4me3 and H3K27ac histone marks at TSS ± 2.5 kb of transcripts that loose (red colored regions in S1E) or gain (blue colored regions in S1E) the H3K27me3 mark during 10 days of osteogenic differentiation (immature-osteoblasts stage). (G and H) Scatter plot of the observed (Obs) to/expected (Exp) ratio indicating the positive correlation between H3K4me3 and gene expression and a negative correlation between H3K27me3 and gene expression (highlighted in red) during osteogenic differentiation.I) GO term analyses of transcripts that lost H3K27me3 and gained H3K4me3 and were induced in expression using DAVID (The Database for Annotation, Visualization and Integrated Discovery, n = 5179). The Y-axis represents the –logP value (with Benjamini correction cut off < 0.05) (J) Genome browser tracks representing ChIP-seq tracks from naive and immature-osteoblasts (10 days of osteogenic differentiation) at gene loci where loss in H3K27me3 and gain in H3K4me3 and H3K27ac at TSS are shown. BMP, Bone Morphogenetic Protein; Lep, Leptin.

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Bone Morphogenetic Protein: The Molecularization of Skeletal System Development

• Authors : Urist, Marshall R.

• Source: Journal of Bone and Mineral Research ; volume 12, issue 3, page 343-346 ; ISSN 0884-0431 1523-4681

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Supplementary file 4. Common genomic regions bound by MYF5 and MYOD in ERMS that comprise the genes found in the ‘embryonic skeletal system development’ module.

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Association of thyroid hormone with osteoarthritis: from mendelian randomization and RNA sequencing analysis.

• Authors : Li, Chengxin¹; Tu, Yucheng¹; Rong, Rong²

• Source: Journal of Orthopaedic Surgery & Research. 7/25/2024, Vol. 19 Issue 1, p1-11. 11p.

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Integrating miRNA and full-length transcriptome profiling to elucidate the mechanism of muscle growth in Muscovy ducks reveals key roles for miR-301a-3p/ANKRD1.

• Authors : Huang, Jiangnan¹ (AUTHOR); Xiong, Xiaolan¹ (AUTHOR); Zhang, Weihong¹ (AUTHOR)

• Source: BMC Genomics. 4/4/2024, Vol. 25 Issue 1, p1-17. 17p.

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