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Pharmacological Inhibition of the Spliceosome SF3b Complex by Pladienolide‐B Elicits Craniofacial Developmental Defects in Mouse and Zebrafish

• Authors : Yukiko Hoshino; Shujie Liu; Toshiko Furutera

Subjects: Mice, Knockout; 0303 health sciences; Apoptosis

• Source: Birth Defects Res

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The SF3b complex: splicing and beyond

• Authors : Chengfu Sun

Subjects: 0301 basic medicine; 03 medical and health sciences; Multiprotein Complexes

• Source: Cell Mol Life Sci

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Structural and mechanistic insights into human splicing factor SF3b complex derived using an integrated approach guided by the cryo-EM density maps

• Authors : Narayanaswamy Srinivasan; Agnel Praveen Joseph; Ramachandra M. Bhaskara

Subjects: Models, Molecular; 0301 basic medicine; 03 medical and health sciences

• Source: RNA Biology. 13:1025-1040

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H3B-8800, an Orally Bioavailable Modulator of the SF3b Complex, Shows Efficacy in Spliceosome-Mutant Myeloid Malignancies

• Authors : John Q. Wang; Eunice Park; Betty Chan

Subjects: 03 medical and health sciences; 0302 clinical medicine; 3. Good health

• Source: Blood. 128:966-966

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Splicing factors of the SF3b complex PHF5A and SF3B1 regulate the DNA damage response in ALL

• Authors : Moorthy, S; Bomken, S; Ponthan, F

• Source: Klinische Pädiatrie ; 32. Jahrestagung der Kind-Philipp-Stiftung für pädiatrisch onkologische Forschung ; ISSN 1439-3824

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CtIP-mediated Alternative mRNA Splicing Fine-tunes the DNA Damage Response

• Authors : Prados Carvajal, Rosario; Rodríguez Real, Guillermo; Gutiérrez Pozo, Gabriel ...

Subjects: CtIP; DNA damage response; MRNA splicing

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Rewards of divergence in sequences, 3-D structures and dynamics of yeast and human spliceosome SF3b complexes

• Authors : Arangasamy Yazhini; Sankaran Sandhya; Narayanaswamy Srinivasan

Subjects: 0303 health sciences; 03 medical and health sciences; Spliceosome

• Source: Curr Res Struct BiolCurrent Research in Structural Biology, Vol 3, Iss, Pp 133-145 (2021)

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CtIP-mediated alternative mRNA splicing fine-tunes the DNA damage response

• Authors : Guillermo Rodríguez-Real; Pablo Huertas; Gabriel Gutierrez-Pozo ...

Subjects: 0301 basic medicine; MRNA splicing; DNA damage response

• Source: RNARISalud-ANDALUCIA. Repositorio Institucional de Salud de AndalucíainstnameDigital.CSIC. Repositorio Institucional del CSICConsejo Superior de Investigaciones Científicas

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Figure 2—figure supplement 1. Characterization of SF3B1 as a CYCLOPS gene. ; (A) Growth of SF3B1gain, SF3B1control-Cal51-2 and SF3B1Loss-Cal51-2, and SF3B1K666N mutant cells (measured as change in light units; Figure 2A) after infection with shRNAs targeting LacZ or SF3B1. (B) Quantitative RT-PCR of SF3B1 expression without (black) or with (red) doxycycline-induced shSF3B1 expression using TR-shSF3B1 #5 demonstrating greater than 85% knockdown. (C) Growth of non-transformed SF3B1neutral cell lines without (black) or with (red) doxycycline-induced shSF3B1 expression using TR-shSF3B1 #5. Growth curves are measured as changes in CellTiterGlo luminescence relative to day 0 of doxycycline treatment. (D) SF3B1 immunoblot from SF3B1neutral and SF3B1loss cells expressing shLacZ (c) or shSF3B1#3 (sh3) hairpins performed in parallel to growth assays in Figure 2A. (E) Ratio of GFP-expressing cells to uninfected controls in SF3B1neutral and SF3B1loss breast and hematopoietic cell lines expressing shLacZ-GFP (black) or shSF3B1#4-GFP (red). (F) Heatmap of False Discovery Rate q-values indicating the significance of associations between copy numbers of SF3b complex members (rows) and sensitivity of those cells to suppression of SF3b complex members by shRNA (columns). (G) PHF5A immunoblot three days after infection with shLacZ or shPHF5A targeting hairpins from HCC1954 cells profiled in panel Figure 2—figure supplement 1G. (H) Growth of breast cancer cell lines expressing shLacZ (black) or shPHF5A (red), measured as changes in CellTiterGlo luminescence relative the day of infection. For all panels, error bars represent ± SD from at least three technical replicates.

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CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

• Authors : Haroon Butt; Ayman Eid; Afaque A. Momin

Subjects: Directed evolution; Genome engineering; Spliceosome

• Source: Genome Biology, Vol 20, Iss 1, Pp 1-9 (2019)

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