نتائج البحث

In Vivo Dopamine Neuron Imaging-Based Small Molecule Screen Identifies Novel Neuroprotective Compounds and Targets

• Authors : Kim, Gha-hyun J; Mo, Han; Liu, Harrison

Subjects: Pharmacology and Pharmaceutical Sciences; Biomedical and Clinical Sciences; Brain Disorders

تفاصيل العنوان

IFT46 gene promoter-driven ciliopathy disease model in zebrafish

• Authors : Mi-Sun Lee; Hye-Jeong Han; Tae-Ik Choi

Subjects: IFT46; ciliopathy; GAL4/UAS system

• Source: Frontiers in Cell and Developmental Biology, Vol 11 (2023)

تفاصيل العنوان

Figure 1—figure supplement 1. Metronidazole-ablated vmhc:mCherry-NTR ventricles display initial cardiomyocyte death, loss of ventricular tissue and impairment of contractile function but later exhibit cardiomyocyte proliferation and recovery of ventricular tissue and contractile function. ; Following ventricular ablation, ventricular and atrial cardiomyocyte proliferation leads to cardiac recovery at 96 hpt. (A, B) Confocal maximum intensity projections of Tg(vmhc:mCherry-NTR) fish show extensive cell death as detected by TUNEL staining in the ventricles of (B) MTZ-treated fish when compared with (A) DMSO-treated controls at 24 hpt. (C) Quantitation of TUNEL-positive CMs in the ventricle of control (n = 15) and ablated (n = 13) fish hearts at 24 hpt confirms cell death is induced by MTZ treatment. (D, E) Confocal maximum intensity projections of Tg(cmlc2:GFP; vmhc:mCherry-NTR) fish show increased proliferation of CMs as detected by EdU+/cmlc2:GFP+ double positive staining (white arrows) in the ventricles and atria of (E) MTZ-treated fish when compared with (D) DMSO-treated controls at 48 hpt. Yellow arrows point to EdU+ proliferating non-cardiomyocytes. (D’, E’) Single confocal sections show EdU+ proliferating cells. (F) Quantitation of EdU+/cmlc2:GFP+ double positive CMs in the ventricle or atrium of control (n = 12) and ablated (n = 12) fish hearts at 48 hpt confirms increased proliferation in ventricle-ablated hearts. (G) Quantitation of ventricular area shows loss of myocardial ventricular tissue in Tg(vmhc:mCherry-NTR) MTZ-treated fish at 24 hpt (n = 12) when compared to control non-ablated fish (n = 7). At 96 hpt, Tg(vmhc:mCherry-NTR) MTZ-ablated hearts show recovery of ventricular area (n = 8, control; n = 9, ablated). (H) Quantitation of ventricular fractional area change (FAC) reveals decreased ventricular function in Tg(vmhc:mCherry-NTR) MTZ-treated fish at 24 hpt (n = 9) when compared to control non-ablated fish (n = 6). At 96 hpt, Tg(vmhc:mCherry-NTR) MTZ-ablated hearts (n = 5) dispaly comparable ventricular function to that of control hearts (n = 6). V, ventricle; A, atrium; dpf, days post-fertilization. Dashed lines outline the heart. Bars: 50 μm. (C) Mean + s.d. (F–H) Mean + s.e.m. Student’s t-test, ***, p0.05.

تفاصيل العنوان

In Vivo Dopamine Neuron Imaging-Based Small Molecule Screen Identifies Novel Neuroprotective Compounds and Targets

• Authors : Gha-hyun J. Kim; Han Mo; Harrison Liu

Subjects: neurodegeneration; NTR-MTZ; aloperine

• Source: Frontiers in Pharmacology, Vol 13 (2022)

تفاصيل العنوان

Figure 1—figure supplement 1. Characterization of radial glia or neuronal ablation by the NTR/Mtz-mediated cell ablation method. ; (A and B) 30 hpf Tg(elavl3:EGFP);TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR) trunk sections at the level of the anterior (A) or posterior (B) SC. EGFP+ neurons and mCherry+ radial glia are largely segregated. Scale bar, 20 µm. (C) Experimental time course of NTR/Mtz-mediated ablation for the panels (D–I”). (D–D” and E–E”) 82 hpf Tg(elavl3:EGFP);Tg(elavl3:gal4);Tg(UAS:mcherry-NTR) trunks after treatment with DMSO (D–D”) or 2 mM Mtz (E–E”) starting at 30 hpf. Unlike DMSO-treated fish that show strong co-expression of Tg(elavl3:EGFP) and Tg(elavl3:gal4);Tg(UAS:mCherry-NTR) in their spinal cord, Mtz-treated fish show a dramatic reduction of this co-expression. (F–F” and G–G”) 82 hpf TgBAC(gfap:gfap-EGFP);Tg(elavl3:gal4);Tg(UAS:mcherry-NTR) trunks after treatment with DMSO (F–F”) or 2 mM Mtz (G–G”) starting at 30 hpf. Mtz-treated fish show a dramatic reduction of Tg(elavl3:gal4);Tg(UAS:mCherry-NTR) expression in their spinal cord, however, TgBAC(gfap:Gfap-EGFP) expression appears unaffected as compared to DMSO-treated fish. (H–H” and I–I”) 82 hpf Tg(elavl3:EGFP);TgBAC(gfap:gal4ff);Tg(UAS:mcherry-NTR) trunks after treatment with DMSO (H–H”) or 2 mM Mtz (I–I”) starting at 30 hpf. Mtz-treated fish show a dramatic reduction of TgBAC(gfap:gal4ff);Tg(UAS:mCherry-NTR) expression in their spinal cord, and Tg(elavl3:EGFP) expression is also slightly reduced as compared to DMSO-treated fish that show strong expression of both TgBAC(gfap:gal4ff);Tg(UAS:mCherry-NTR) and Tg(elavl3:EGFP) expression in their spinal cord. Scale bar, 100 µm.

تفاصيل العنوان

Repurposing renin angiotensin pathway inhibitors as disease modifying therapeutics for Parkinson’s Disease

• Authors : Kim, Gha-hyun Jeffrey

Subjects: Pharmaceutical sciences; Neurosciences; Molecular biology

تفاصيل العنوان

DataSheet1_IFT46 gene promoter-driven ciliopathy disease model in zebrafish.docx

• Authors : Mi-Sun Lee; Hye-Jeong Han; Tae-Ik Choi

Subjects: Cell Biology; Marine Biology; Cell Development

تفاصيل العنوان

P0053A METHOD FOR THE ISOLATION OF FLUORESCENT GLOMERULI FROM ZEBRAFISH LARVAE

• Authors : Iervolino, Anna; Lange, Tim; Siegerist, Florian

• Source: Nephrology Dialysis Transplantation ; volume 35, issue Supplement_3 ; ISSN 0931-0509 1460-2385

تفاصيل العنوان

Establishment of a Drug Screening Model for Cardiac Complications of Acute Renal Failure.

• Authors : Liao, Shuyi¹ (AUTHOR) ; Yang, Wenmin¹ (AUTHOR) ; Yu, Ting² (AUTHOR)

• Source: Biomolecules (2218-273X). Sep2021, Vol. 11 Issue 9, p1370-1370. 1p.

تفاصيل العنوان

mTORC1 signaling pathway regulates tooth repair

• Authors : Honghong Liu; Yu Yue; Zhiyun Xu

Subjects: Dentistry; RK1-715

• Source: International Journal of Oral Science, Vol 15, Iss 1, Pp 1-12 (2023)

تفاصيل العنوان