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Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca2+

• Authors : Wei-Yin Lin; Woo Young Chung; Seonghee Park

Subjects: Science

• Source: Nature Communications, Vol 16, Iss 1, Pp 1-20 (2025)

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Super-resolution insights into the role of Esyt1 in ER-PM junction dynamics in sensory neurons

• Authors : Palfrey, Char; Gamper, Nikita; Shah, Shihab S.

• Source: Biophysical Journal ; volume 123, issue 3, page 172a ; ISSN 0006-3495

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ER-PM Junction Proteins and Their Roles in Regulating Cell Homeostasis and Signaling

• Authors : Quintanilla, Carlo Giovanni;

Subjects: Endoplasmic Reticulum; Membrane Proteins

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Localization of the tubby domain, a PI(4,5)P 2 biosensor, to E-Syt3-rich endoplasmic reticulum–plasma membrane junctions

• Authors : Thallmair, Veronika; Schultz, Lea; Evers, Saskia

Subjects: ER–PM junction; Fluorescence imaging; Lipid-binding domain

• Source: Thallmair, V, Schultz, L, Evers, S, Jolie, T, Goecke, C, Leitner, M G, Thallmair, S & Oliver, D 2023, 'Localization of the tubby domain, a PI(4,5)P 2 biosensor, to E-Syt3-rich endoplasmic

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ER-PM Junctions on GABAergic Interneurons Are Organized by Neuregulin 2/VAP Interactions and Regulated by NMDA Receptors

• Authors : Detlef Vullhorst; Mara S. Bloom; Neha Akella

Subjects: ER-PM junction; neuregulin 2; GABAergic interneuron

• Source: International Journal of Molecular Sciences, Vol 24, Iss 3, p 2908 (2023)

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Figure 1—figure supplement 5. nAbs that function as intrabodies against inhibitory synaptic and ER-PM junction target proteins localize to their respective subcellular domains in cultured hippocampal neurons. ; Representative images of the colabeling for proteins related to targets of nAbs and expressed nAbs-EGFP in neurons. (A) Each row shows representative images of a nAb-EGFP fusions (green) against the inhibitory synaptic protein Gephyrin (top row) or the ER-PM junction protein AMIGO-1 (second row) and in magenta immunolabeling with mAbs that label synapses (the pan-synapsin mAb L125/129 that sees all synapsin isoforms,) or Kv2 channel-containing ER-PM junctions (mAb K89/34 against Kv2.1 to label. The scale bar in the top left panel is 5 μm and holds for all panels in figure except the magnified insets, for which the scale bar is 2 μm. Panels to the far right of each row are the normalized fluorescence intensity values across the individual line scans from the magnified inset, with the corresponding R2 values. Note the concordance of the intensity values for the nAb-EGFP fusions (green) with labeling for the compartment-specific markers (magenta). (B) Graph of PCC values between the different nAb-GFP fusions and EGFP with immunolabeling for synapses (L125/129) or ER-PM junctions (K89/34). (C) Size analysis of mAb labeled puncta. Left: synapses (labeled with anti-pan-synapsin mAb L125/126) in anti-Gephyrin nAb-EGFP transfected and untransfected cells: ns, p=0.7063. Right: Kv2.1-containing ER-PM junctions (labeled with anti-Kv2.1 mAb K89/34) in anti-AMIGO-1 nAb-EGFP transfected and untransfected cells: ns, p=0.6513. Two-tailed unpaired t-tests. Bars on all graphs are mean ± SD.

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ER-PM Junctions on GABAergic Interneurons Are Organized by Neuregulin 2/VAP Interactions and Regulated by NMDA Receptors.

• Authors : Vullhorst, Detlef¹ (AUTHOR) ; Bloom, Mara S.¹ (AUTHOR); Akella, Neha¹ (AUTHOR)

Subjects: *METHYL aspartate receptors; *INTERNEURONS; *NEUREGULINS

• Source: International Journal of Molecular Sciences. Feb2023, Vol. 24 Issue 3, p2908. 23p.

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Figure 1—figure supplement 3. nAbs that function as intrabodies against inhibitory synaptic and ER-PM junction target proteins colocalize with endogenously expressed target proteins in cultured hippocampal neurons. ; Representative images of intrabody-positive nAbs bound to endogenous targets after expression in cultured hippocampal neurons. (A) The top row shows the diffuse localization of EGFP in the dendrite (left) and soma (right) of an EGFP-transfected neuron. The subsequent rows show neurons expressing nAb-EGFP fusions and showing EGFP fluorescence (green), target-specific mAb labeling (magenta), and the merged image, with an adjacent panel showing a magnified image of the inset marked by the dashed box. The scale bar in the top left panel is 5 μm and holds for all panels in figure except the magnified insets, for which the scale bar is 2 μm. Panels to the far right of each row are the normalized fluorescence intensity values across the individual line scans from the magnified inset, with the corresponding R2 values. Note the concordance of the nAb (green) and anti-target mAb (magenta) intensity values. (B) Graph of PCC values between nAb-EGFP fusions and anti-target mAb labeling (Gephyrin: L106/23; AMIGO-1: L86A/37). PCC values of Gephyrin and AMIGO-1 mAb immunolabeling with EGFP fluorescence in cells expressing EGFP are also shown. (C) Size analysis of mAb labeled puncta of target proteins between nAb-EGFP transfected and untransfected cells. Gephyrin: ns, p=0.8758; AMIGO-1: ns, p=0.1091; two-tailed unpaired t-tests. Bars on all graphs are mean ± SD.

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The Builders of the Junction: Roles of Junctophilin1 and Junctophilin2 in the Assembly of the Sarcoplasmic Reticulum–Plasma Membrane Junctions in Striated Muscle

• Authors : Perni, S.; Perni, S.

Subjects: striated muscle; ER-PM junction; junctophilin

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Electrophysiological analysis of Kv2 channel regulation by non-canonical and canonical mechanisms

• Authors : Maverick, Emily E., author; Tamkun, Michael, advisor; Amberg, Gregory, committee member

Subjects: ion channel; ER-PM junction; Kv2

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