Source: BMC veterinary research [BMC Vet Res] 2024 Dec 19; Vol. 20 (1), pp. 568. Date of Electronic Publication: 2024 Dec 19.Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148

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Academic Journal

Laboratory validation of a simplified DNA extraction protocol followed by a portable qPCR detection of M. tuberculosis DNA suitable for point of care settings.

Authors : Soares TDS; Laboratório de Biologia Molecular, ULBRA, Canoas, RS, Brasil.; Centro de Desenvolvimento Científico e Tecnológico (CDCT)-Centro Estadual de Vigilância em Saúde (CEVS)-Secretaria do Estado do Rio Grande do Sul (SES/ RS), Porto Alegre, RS, Brasil.

Subjects: Mycobacterium tuberculosis*/Mycobacterium tuberculosis*/Mycobacterium tuberculosis*/genetics ; Mycobacterium tuberculosis*/Mycobacterium tuberculosis*/Mycobacterium tuberculosis*/isolation & purification ; DNA, Bacterial*/DNA, Bacterial*/DNA, Bacterial*/isolation & purification

Source: PloS one [PLoS One] 2024 Dec 16; Vol. 19 (12), pp. e0302345. Date of Electronic Publication: 2024 Dec 16 (Print Publication: 2024).Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet

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Academic Journal

Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite.

Authors : Mijnendonckx K; Unit of Microbiology, Belgian Nuclear Research Centre, Mol, Belgium.; Smolders C

Subjects: Bentonite*/Bentonite*/Bentonite*/chemistry ; RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/genetics ; DNA, Bacterial*/DNA, Bacterial*/DNA, Bacterial*/genetics

Source: Environmental microbiology reports [Environ Microbiol Rep] 2024 Dec; Vol. 16 (6), pp. e70047.Publisher: Society for Applied Microbiology and Blackwell Pub Country of Publication: United States NLM ID: 101499207 Publication Model: Print Cited Medium:

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Academic Journal

Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery.

Authors : Kruasuwan W; Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.; Siriraj Long-read Lab (Si-LoL), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Subjects: Genome, Bacterial* ; Plasmids*/Plasmids*/Plasmids*/genetics ; DNA, Bacterial*/DNA, Bacterial*/DNA, Bacterial*/genetics

Source: Scientific reports [Sci Rep] 2024 Nov 12; Vol. 14 (1), pp. 27672. Date of Electronic Publication: 2024 Nov 12.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN:

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Comparison of DNA extraction procedures for detection of Mycoplasma bovis directly from extended bovine semen straw samples using a commercial M. bovis PCR.

Authors : Taylor E; Mycoplasma Team, Department of Bacteriology, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK. .; Deeney A

Subjects: Mycoplasma bovis*/Mycoplasma bovis*/Mycoplasma bovis*/isolation & purification ; Mycoplasma bovis*/Mycoplasma bovis*/Mycoplasma bovis*/genetics ; Semen*/Semen*/Semen*/microbiology

Source: BMC veterinary research [BMC Vet Res] 2024 Oct 26; Vol. 20 (1), pp. 491. Date of Electronic Publication: 2024 Oct 26.Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148

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Academic Journal

Reconstruction of a robust bacterial replication module.

Authors : Wang T; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences/Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, PR China.; He F

Subjects: DNA Replication*/DNA Replication*/DNA Replication*/genetics ; Escherichia coli*/Escherichia coli*/Escherichia coli*/genetics ; Escherichia coli*/Escherichia coli*/Escherichia coli*/growth & development

Source: Nucleic acids research [Nucleic Acids Res] 2024 Oct 14; Vol. 52 (18), pp. 11394-11407.Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN:

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Assessment of Skimmed Milk Flocculation for Bacterial Enrichment from Water Samples, and Benchmarking of DNA Extraction and 16S rRNA Databases for Metagenomics.

Authors : Donchev D; National Reference Laboratory for Control and Monitoring of Antimicrobial Resistance, Department of Microbiology, National Center of Infectious and Parasitic Diseases, 26 Yanko Sakazov Blvd., 1504 Sofia, Bulgaria.; Stoikov I

Subjects: RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/genetics ; Milk*/Milk*/Milk*/microbiology ; Metagenomics*/Metagenomics*/Metagenomics*/methods

Source: International journal of molecular sciences [Int J Mol Sci] 2024 Oct 08; Vol. 25 (19). Date of Electronic Publication: 2024 Oct 08.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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Academic Journal

Validation of the performance of a point of care molecular test for leprosy: From a simplified DNA extraction protocol to a portable qPCR.

Authors : Bertão-Santos A; Laboratório de Ciências e Tecnologias Aplicadas à Saúde (LaCTAS), Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Curitiba, Brazil.; Dias LDS

Subjects: Mycobacterium leprae*/Mycobacterium leprae*/Mycobacterium leprae*/genetics ; Mycobacterium leprae*/Mycobacterium leprae*/Mycobacterium leprae*/isolation & purification ; Leprosy*/Leprosy*/Leprosy*/diagnosis

Source: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 Oct 07; Vol. 18 (10), pp. e0012032. Date of Electronic Publication: 2024 Oct 07 (Print Publication: 2024).Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet

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Academic Journal

Integrative Biomarker Panel for Improved Lung Cancer Diagnosis Using Plasma microRNAs and Sputum Bacterial DNA.

Authors : Dhilipkannah P; Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201-1192, USA.; Sachdeva A

Subjects: Lung Neoplasms*/Lung Neoplasms*/Lung Neoplasms*/blood ; Lung Neoplasms*/Lung Neoplasms*/Lung Neoplasms*/diagnosis ; Lung Neoplasms*/Lung Neoplasms*/Lung Neoplasms*/genetics

Source: Current oncology (Toronto, Ont.) [Curr Oncol] 2024 Oct 02; Vol. 31 (10), pp. 5949-5959. Date of Electronic Publication: 2024 Oct 02.Publisher: MDPI Country of Publication: Switzerland NLM ID: 9502503 Publication Model: Electronic Cited Medium: Internet ISSN: 1718-7729

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Academic Journal

Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing.

Authors : Baer M; Institute of Nutritional and Food Sciences, Food Microbiology and Hygiene, University of Bonn, Friedrich-Hirzebruch-Allee 7, 53115, Bonn, Germany. .; Höppe L

Subjects: RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/genetics ; DNA, Bacterial*/DNA, Bacterial*/DNA, Bacterial*/genetics ; Polymerase Chain Reaction*/Polymerase Chain Reaction*/Polymerase Chain Reaction*/methods

Source: BMC microbiology [BMC Microbiol] 2024 Dec 06; Vol. 24 (1), pp. 521. Date of Electronic Publication: 2024 Dec 06.Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180

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نتائج البحث

فلترة النتائج

Shaken, not stirred: magnetic bead DNA extraction as a rapid and effective method for the scaling up of bovine tuberculosis diagnosis.

Laboratory validation of a simplified DNA extraction protocol followed by a portable qPCR detection of M. tuberculosis DNA suitable for point of care settings.

Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite.

Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery.

Comparison of DNA extraction procedures for detection of Mycoplasma bovis directly from extended bovine semen straw samples using a commercial M. bovis PCR.

Reconstruction of a robust bacterial replication module.

Assessment of Skimmed Milk Flocculation for Bacterial Enrichment from Water Samples, and Benchmarking of DNA Extraction and 16S rRNA Databases for Metagenomics.

Validation of the performance of a point of care molecular test for leprosy: From a simplified DNA extraction protocol to a portable qPCR.

Integrative Biomarker Panel for Improved Lung Cancer Diagnosis Using Plasma microRNAs and Sputum Bacterial DNA.

Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing.

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