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Competing Interactions between Viral RHIM Amyloid-forming Proteins and Host Functional Amyloid Structures Modulate the Cellular Response to Infection

• Authors : Sunder, M; Pham, CLL; Shanmugam, N

Subjects: 02 Physical Sciences; 03 Chemical Sciences; 06 Biological Sciences

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Competing Interactions between Viral RHIM Amyloid-forming Proteins and Host Functional Amyloid Structures Modulate the Cellular Response to Infection

• Authors : Sunde, Margaret; Pham, Chi LL; Shanmugam, Nirukshan

Subjects: anzsrc-for: 02 Physical Sciences; anzsrc-for: 03 Chemical Sciences; anzsrc-for: 06 Biological Sciences

• Source: urn:ISSN:0006-3495 ; urn:ISSN:1542-0086 ; 64th Annual Meeting of the Biophysical-Society, CA, San Diego, 2020-02-15 - 2020-02-19

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The Central Conserved Region (CCR) of Respiratory Syncytial Virus (RSV) G Protein Modulates Host miRNA Expression and Alters the Cellular Response to Infection

• Authors : Abhijeet A. Bakre; Jennifer L. Harcourt; Lia M. Haynes

Subjects: respiratory syncytial virus; RSV; microRNAs

• Source: Vaccines, Vol 5, Iss 3, p 16 (2017)

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The central conserved region (CCR) of respiratory syncytial virus (RSV) G protein modulates host miRNA expression and alters the cellular response to infection

• Authors : Bakre, Abhijeet A.; Harcourt, Jennifer L.; Haynes, Lia M.

• Source: Bakre , A A , Harcourt , J L , Haynes , L M , Anderson , L J & Tripp , R A 2017 , ' The central conserved region (CCR) of respiratory syncytial virus (RSV) G protein modulates host miRNA expression

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The mucosal cellular response to infection with Ancylostoma ceylanicum

• Authors : Alkazmi, L.M.M.; Dehlawi, M.S.; Behnke, J.M.

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The Herpes Simplex Virus Type 1 vhs-UL41 Gene Secures Viral Replication by Temporarily Evading Apoptotic Cellular Response to Infection: Vhs-UL41 Activity Might Require Interactions with Elements of Cellular mRNA Degradation Machinery

• Authors : Barzilai, Ari; Zivony-Elbom, Ifaat; Sarid, Ronit

• Source: Journal of Virology ; volume 80, issue 1, page 505-513 ; ISSN 0022-538X 1098-5514

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Figure 3. Analysis of Toxoplasma gondii infection in IFNγ-treated HeLa cells. ; HeLa cells were stimulated with 100 IU/mL IFNγ, infected with type I (RH) Toxoplasma gondii (Tg) and analyzed 6 hr post-infection. (A) Infection parameters depicted as total percent of Tg infected cells, the ratio of Tg vacuoles to cells and the ratio of parasites to cells. (B) Cellular readouts showing the proportion of cells that contain a varying numbers of parasite vacuoles, the mean vacuole size of Tg and the vacuole position as the value of the mean Euclidian distance of Tg vacuoles to the host cell nucleus. (C) Replication capacity of Tg shown as the proportion of replicating parasites and the distribution of replicating Tg. (D) Cellular response to infection with Tg measured as the percentage of cells that decorate vacuoles and the average proportion of vacuoles per cell that are being decorated simultaneously and the overall proportion of ubiquitin and/or p62 decorated Tg vacuoles. N shows the total number of vacuoles analyzed for each condition, percentages are indicated in the legend. (E) Properties of the host protein coat on Tg vacuoles as the average coat distance for ubiquitin and p62 to Tg and mean fluorescence intensity of ubiquitin and p62 at Tg vacuoles. (F) Fate of Tg vacuoles grouped based on host protein recruitment. The proportion of replicating parasites and the replication distribution based on recruitment status of the vacuole are shown. All data shown above represent the mean of N = 3 experiments±SEM. Significance was determined using unpaired t-tests, n.s. = not significant, *p≤0.05; **p≤0.01, ***p≤0.001, ****p≤0.0001.

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Figure 4. Analysis of Salmonella enterica Typhimurium infection in IFNγ-treated HeLa cells. ; HeLa cells were stimulated with 100 IU/mL IFNγ, infected with Salmonella enterica Typhimurium (STm) and analyzed 2 hr post-infection. (A–C) Stage one infection analysis parameters. (A) Infection parameters depicted as total percent of STm infected cells, the ratio of STm vacuoles to cells and the ratio of bacteria to cells. (B) Cellular readouts showing the proportion of cells that contain a certain number of bacteria vacuoles, the mean vacuole size of STm and the vacuole position as the value of the mean euclidian distance of STm vacuoles to the host cell nucleus. (C) Replication capacity of STm shown as the proportion of replicating bacteria and the distribution of replicating STm. (D) Training of the deep convolution neural network (CNN) to analyze host protein recruitment to STm vacuoles and bacteria. Left: Example images showing the difference of no recruitment versus ubiquitin (magenta) recruitment to STm. Middle: Decrease of negative log likelihood (NLL) used as loss function during CNN training over training cycles (epochs) for STm model. Right: Confusion matrix of STm model validation, classification accuracy (0 to 1) during validation is colour-coded blue to red and indicated in the figure. (E) Cellular response to infection with STm measured through the percentage of cells that decorate vacuoles and the average proportion of vacuoles per cell that are being decorated simultaneously and the overall proportion of ubiquitin decorated STm vacuoles. N shows the total number of vacuoles analyzed for each condition, percentages are indicated in the legend. (F) Properties of the host protein coat on STm vacuoles as the average coat distance for ubiquitin to STm and mean fluorescence intensity of ubiquitin. (G) Fate of STm grouped based on host protein recruitment. Shown is the proportion of replicating bacteria and the replication distribution based on recruitment status of the vacuole. All data shown above represent the mean of N = 3 experiments±SEM. Significance was determined using unpaired t-tests, n.s. = not significant, *p≤0.05; **p≤0.01, ***p≤0.001, ****p≤0.0001.

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The mucosal cellular response to infection with Ancylostoma ceylanicum

• Authors : Alkazmi, L.M.M.; Dehlawi, M.S.; Behnke, J.M.

Subjects: Article; PeerReviewed

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The mucosal cellular response to infection with Ancylostoma ceylanicum

• Authors : Alkazmi, L.M.M.; Dehlawi, M.S.; Behnke, J.M.

Subjects: Article; PeerReviewed

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