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(A–D) Testing the reproducibility of the odor stimulus. This initial test was performed with 2 ml undiluted 2-heptanone placed in a 10 ml glass bottle. Air dilutions of the odor vapor were performed and delivered as described in Materials and methods. (A) A Photo Ionization Detector (PID, Aurora) was used to measure the odor stimulus produced by the random switching of the valve. The random sequence was applied for 1 min every 10 min 14 times (color coded). In between recordings the odor stream was directed to waste. (B) Maximum PID signal reached within the duration of the first odor pulse. The odor stimulus decays during the first 20 min of stimulation. (C) Scatterplot of the maximum PID signal calculated within each odor pulse for two stimuli delivered consecutively (color indicates consecutive 1 min long stimulus sequences). Data points on the black line indicate that the consecutive odor stimuli were identical. (D) Maximum PID response reached for the corresponding pulse duration. Please note that the stimuli used for the imaging had minimum pulse duration of 300 ms (arrow). (A’–D’) Same as (A-D) for measurements conducted on a different day after waiting 20 min for odor equilibration. (E) Mean PID signal in response to methyl acetate at 3 values of the air dilutions (C5, C8, C13, tested in random order). Shaded areas indicate SEM (n=5). (F) Mean PID response (± SEM) to the first pulse in the random sequence as a function of the gas dilution. The linear fit (black line) indicates a linear relationship between the applied odor concentration (gas dilution) and the PID readout

Subjects: nervous system; D. melanogaster; LN model

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  • 1-10 ل  8,269 نتائج ل ""∼ 14 times""