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Academic Journal

A novel KRAS exon 2 drop-off digital PCR assay for mutation detection in cell-free DNA of cancer patients.

Authors : Addamo-De Nard B; Department of Medical Oncology and Hematology, University Hospital Zurich, Zurich, Switzerland.; Geissmann M

Subjects: Proto-Oncogene Proteins p21(ras)*/Proto-Oncogene Proteins p21(ras)*/Proto-Oncogene Proteins p21(ras)*/genetics ; Mutation* ; Circulating Tumor DNA*/Circulating Tumor DNA*/Circulating Tumor DNA*/genetics

Source: Diagnostic pathology [Diagn Pathol] 2025 May 24; Vol. 20 (1), pp. 62. Date of Electronic Publication: 2025 May 24.Publisher: BioMed Central Country of Publication: England NLM ID: 101251558 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-1596

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Academic Journal

Performance Comparison of Droplet Digital PCR and Next-Generation Sequencing for Circulating Tumor DNA Detection in Non-Metastatic Rectal Cancer.

Authors : Szeto S; Applied Tumor Genomics Research Program, Research Program Unit, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Kytölä S

Subjects: Rectal Neoplasms*/Rectal Neoplasms*/Rectal Neoplasms*/genetics ; Rectal Neoplasms*/Rectal Neoplasms*/Rectal Neoplasms*/blood ; Rectal Neoplasms*/Rectal Neoplasms*/Rectal Neoplasms*/diagnosis

Source: Cancer medicine [Cancer Med] 2025 May; Vol. 14 (9), pp. e70943.Publisher: John Wiley & Sons Ltd Country of Publication: United States NLM ID: 101595310 Publication Model: Print Cited Medium: Internet ISSN:

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Academic Journal

Establishment of a simple prediction method for DNA melting temperature: high-resolution melting curve analysis of PCR products.

Authors : Zhou Y; Hainan Provincial Tropical Forensic Engineering Research Center & Hainan Provincial Academician Workstation (Tropical Forensic Medicine), Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, China.; Ha S

Subjects: DNA*/DNA*/DNA*/chemistry ; DNA*/DNA*/DNA*/genetics ; Nucleic Acid Denaturation*

Source: PloS one [PLoS One] 2025 Apr 16; Vol. 20 (4), pp. e0321885. Date of Electronic Publication: 2025 Apr 16 (Print Publication: 2025).Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet

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Academic Journal

Enhancing trace DNA profile recovery in forensic casework using the amplicon RX post-PCR clean-up kit.

Authors : Aljanahi NS; The Biology and DNA Section, General Department of Forensic Science and Criminology, Dubai Police General Head Quarters, Dubai, United Arab Emirates.; Alketbi SK

Subjects: DNA*/DNA*/DNA*/genetics ; DNA*/DNA*/DNA*/isolation & purification ; DNA*/DNA*/DNA*/analysis

Source: Scientific reports [Sci Rep] 2025 Jan 27; Vol. 15 (1), pp. 3324. Date of Electronic Publication: 2025 Jan 27.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN:

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Academic Journal

A new approach for detecting HPV DNA in cervical swabs: comparison of nucleic acid extraction with direct PCR.

Authors : Çubuk F; National HPV Laboratory, Department of National Reference Laboratories and Biological Products, Public Health General Directorate, Ministry of Health, Ankara, Türkiye, Turkey.; Department of National Reference Laboratories and Biological Products, Public Health General Directorate, Ministry of Health, Ankara, Türkiye, Turkey.

Subjects: Papillomavirus Infections*/Papillomavirus Infections*/Papillomavirus Infections*/diagnosis ; Papillomavirus Infections*/Papillomavirus Infections*/Papillomavirus Infections*/virology ; DNA, Viral*/DNA, Viral*/DNA, Viral*/isolation & purification

Source: Virology journal [Virol J] 2025 Jan 11; Vol. 22 (1), pp. 8. Date of Electronic Publication: 2025 Jan 11.Publisher: BioMed Central Country of Publication: England NLM ID: 101231645 Publication Model: Electronic Cited Medium: Internet ISSN: 1743-422X

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Academic Journal

Digital droplet PCR-based detection and quantification of ovine papillomavirus DNA from the vaginal virobiota of healthy mares.

Authors : Cutarelli A; Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy.; Passantino G

Subjects: Vagina*/Vagina*/Vagina*/virology ; DNA, Viral*/DNA, Viral*/DNA, Viral*/genetics ; DNA, Viral*/DNA, Viral*/DNA, Viral*/analysis

Source: Scientific reports [Sci Rep] 2025 Mar 22; Vol. 15 (1), pp. 9951. Date of Electronic Publication: 2025 Mar 22.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN:

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Academic Journal

Impact of Bridged Nucleic Acid Positions within Blocking Oligonucleotides on DNA Amplification Inhibition in Wild-Type Blocking PCR.

Authors : Yamashita T; Division of Molecular Target and Gene Therapy Products, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan.; Tsukumo Y

Subjects: Oligonucleotides*/Oligonucleotides*/Oligonucleotides*/chemistry ; Oligonucleotides*/Oligonucleotides*/Oligonucleotides*/genetics ; Polymerase Chain Reaction*/Polymerase Chain Reaction*/Polymerase Chain Reaction*/methods

Source: Biological & pharmaceutical bulletin [Biol Pharm Bull] 2025; Vol. 48 (5), pp. 606-612.Publisher: Pharmaceutical Society of Japan Country of Publication: Japan NLM ID: 9311984 Publication Model: Print Cited Medium: Internet ISSN:

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Academic Journal

DAPE cloning with modified primers for producing designated lengths of 3' single-stranded ends in PCR products.

Authors : Lee S; Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Korea (ROK).; Kim H

Subjects: Exodeoxyribonucleases*/Exodeoxyribonucleases*/Exodeoxyribonucleases*/genetics ; Exodeoxyribonucleases*/Exodeoxyribonucleases*/Exodeoxyribonucleases*/metabolism ; DNA Primers*/DNA Primers*/DNA Primers*/chemistry

Source: PloS one [PLoS One] 2025 Feb 13; Vol. 20 (2), pp. e0318015. Date of Electronic Publication: 2025 Feb 13 (Print Publication: 2025).Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet

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Academic Journal

Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing.

Authors : Baer M; Institute of Nutritional and Food Sciences, Food Microbiology and Hygiene, University of Bonn, Friedrich-Hirzebruch-Allee 7, 53115, Bonn, Germany. .; Höppe L

Subjects: RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/genetics ; DNA, Bacterial*/DNA, Bacterial*/DNA, Bacterial*/genetics ; Polymerase Chain Reaction*/Polymerase Chain Reaction*/Polymerase Chain Reaction*/methods

Source: BMC microbiology [BMC Microbiol] 2024 Dec 06; Vol. 24 (1), pp. 521. Date of Electronic Publication: 2024 Dec 06.Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180

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Academic Journal

Sensitive Detection of Gynecological Cancer Recurrence Using Circulating Tumor DNA and Digital PCR: A Comparative Study with Serum Biochemical Markers.

Authors : Balasan N; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy.; Kharrat F

Subjects: Circulating Tumor DNA*/Circulating Tumor DNA*/Circulating Tumor DNA*/blood ; Circulating Tumor DNA*/Circulating Tumor DNA*/Circulating Tumor DNA*/genetics ; Biomarkers, Tumor*/Biomarkers, Tumor*/Biomarkers, Tumor*/blood

Source: International journal of molecular sciences [Int J Mol Sci] 2024 Nov 08; Vol. 25 (22). Date of Electronic Publication: 2024 Nov 08.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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نتائج البحث

فلترة النتائج

A novel KRAS exon 2 drop-off digital PCR assay for mutation detection in cell-free DNA of cancer patients.

Performance Comparison of Droplet Digital PCR and Next-Generation Sequencing for Circulating Tumor DNA Detection in Non-Metastatic Rectal Cancer.

Establishment of a simple prediction method for DNA melting temperature: high-resolution melting curve analysis of PCR products.

Enhancing trace DNA profile recovery in forensic casework using the amplicon RX post-PCR clean-up kit.

A new approach for detecting HPV DNA in cervical swabs: comparison of nucleic acid extraction with direct PCR.

Digital droplet PCR-based detection and quantification of ovine papillomavirus DNA from the vaginal virobiota of healthy mares.

Impact of Bridged Nucleic Acid Positions within Blocking Oligonucleotides on DNA Amplification Inhibition in Wild-Type Blocking PCR.

DAPE cloning with modified primers for producing designated lengths of 3' single-stranded ends in PCR products.

Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing.

Sensitive Detection of Gynecological Cancer Recurrence Using Circulating Tumor DNA and Digital PCR: A Comparative Study with Serum Biochemical Markers.

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