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Academic Journal

Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing.

Authors : Baer M; Institute of Nutritional and Food Sciences, Food Microbiology and Hygiene, University of Bonn, Friedrich-Hirzebruch-Allee 7, 53115, Bonn, Germany. .; Höppe L

Subjects: RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/RNA, Ribosomal, 16S*/genetics ; DNA, Bacterial*/DNA, Bacterial*/DNA, Bacterial*/genetics ; Polymerase Chain Reaction*/Polymerase Chain Reaction*/Polymerase Chain Reaction*/methods

Source: BMC microbiology [BMC Microbiol] 2024 Dec 06; Vol. 24 (1), pp. 521. Date of Electronic Publication: 2024 Dec 06.Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180

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Academic Journal

ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples.

Authors : Abellan-Schneyder I; Core Facility Microbiome, ZIEL - Institute for Food & Health, Technische Universität München, Freising, Germany.; Schusser AJ

Subjects: Biomass*; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods ; RNA, Ribosomal, 16S/RNA, Ribosomal, 16S/RNA, Ribosomal, 16S/*genetics

Source: BMC microbiology [BMC Microbiol] 2021 Dec 18; Vol. 21 (1), pp. 349. Date of Electronic Publication: 2021 Dec 18.Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180

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Academic Journal

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique.

Authors : Pomastowski P; Interdisciplinary Center for Modern Technologies, Nicolaus Copernicus University in Torun, Torun, Poland.; Złoch M

Subjects: Bacteria/Bacteria/Bacteria/*classification ; Bacterial Typing Techniques/Bacterial Typing Techniques/Bacterial Typing Techniques/*methods ; DNA, Ribosomal/DNA, Ribosomal/DNA, Ribosomal/*analysis

Source: PloS one [PLoS One] 2019 May 23; Vol. 14 (5), pp. e0217078. Date of Electronic Publication: 2019 May 23 (Print Publication: 2019).Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet

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Editorial & Opinion

The 16S rRNA gene-based PCR method used for the detection of segmented filamentous bacteria in the intestinal microbiota generates false-positive results.

Authors : Nowrouzian FL; Department of Infectious Diseases, Institution of Biomedicine, Gothenburg University, Gothenburg, Sweden.; Stadler LS

Subjects: Gastrointestinal Microbiome*/Gastrointestinal Microbiome*/Gastrointestinal Microbiome*/genetics; Bacteria/Bacteria/Bacteria/*isolation & purification ; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods

Source: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2017 Oct; Vol. 125 (10), pp. 940-942.Publisher: Munksgaard Country of Publication: Denmark NLM ID: 8803400 Publication Model: Print Cited Medium: Internet ISSN: 1600-0463

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Academic Journal

A New Primer to Amplify pmoA Gene From NC10 Bacteria in the Sediments of Dongchang Lake and Dongping Lake.

Authors : Wang S; College of Life Science, Liaocheng University, Liaocheng, 252059, People's Republic of China. .; Liu Y

Subjects: Genes, Bacterial* ; Genetic Variation* ; Lakes*

Source: Current microbiology [Curr Microbiol] 2017 Aug; Vol. 74 (8), pp. 908-914. Date of Electronic Publication: 2017 May 13.Publisher: Springer International Country of Publication: United States NLM ID: 7808448 Publication Model: Print-Electronic Cited Medium: Internet

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Academic Journal

Novel micelle PCR-based method for accurate, sensitive and quantitative microbiota profiling.

Authors : Boers SA; Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre Rotterdam (Erasmus MC), Rotterdam, The Netherlands.; Hays JP

Subjects: Microbiota*; Bacteria/Bacteria/Bacteria/*classification ; Bacteria/Bacteria/Bacteria/*genetics

Source: Scientific reports [Sci Rep] 2017 Apr 05; Vol. 7, pp. 45536. Date of Electronic Publication: 2017 Apr 05.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN:

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Academic Journal

Broad-Range Detection of Microorganisms Directly from Bronchoalveolar Lavage Specimens by PCR/Electrospray Ionization-Mass Spectrometry.

Authors : Ullberg M; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.; Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.

Subjects: Bacteria/Bacteria/Bacteria/*genetics ; Bronchoalveolar Lavage Fluid/Bronchoalveolar Lavage Fluid/Bronchoalveolar Lavage Fluid/*microbiology ; DNA, Bacterial/DNA, Bacterial/DNA, Bacterial/*analysis

Source: PloS one [PLoS One] 2017 Jan 13; Vol. 12 (1), pp. e0170033. Date of Electronic Publication: 2017 Jan 13 (Print Publication: 2017).Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet

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Academic Journal

Improved PCR primers to amplify 16S rRNA genes from NC10 bacteria.

Authors : He Z; Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.; Wang J

Subjects: Polymerase Chain Reaction*; Bacteria/Bacteria/Bacteria/*classification ; Bacteria/Bacteria/Bacteria/*genetics

Source: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2016 Jun; Vol. 100 (11), pp. 5099-108. Date of Electronic Publication: 2016 Mar 28.Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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Academic Journal

Decontamination of 16S rRNA gene amplicon sequence datasets based on bacterial load assessment by qPCR.

Authors : Lazarevic V; Genomic Research Laboratory, Department of Genetics and Laboratory Medicine, Department of Medical Specialties, Geneva University Hospitals, Rue Gabrielle-Perret-Gentil 4, CH-1211, Geneva, 14, Switzerland. .; Gaïa N

Subjects: Bacteria/Bacteria/Bacteria/*classification ; Computational Biology/Computational Biology/Computational Biology/*methods ; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods

Source: BMC microbiology [BMC Microbiol] 2016 Apr 23; Vol. 16, pp. 73. Date of Electronic Publication: 2016 Apr 23.Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180

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Academic Journal

Comparative analysis of two 16S rRNA gene-based PCR primer sets provides insight into the diversity distribution patterns of anammox bacteria in different environments.

Authors : Wang S; State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People's Republic of China.; Hong Y

Subjects: Bacteria/Bacteria/Bacteria/*metabolism ; DNA Primers/DNA Primers/DNA Primers/*genetics ; Polymerase Chain Reaction/Polymerase Chain Reaction/Polymerase Chain Reaction/*methods

Source: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2015 Oct; Vol. 99 (19), pp. 8163-76. Date of Electronic Publication: 2015 Aug 01.Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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نتائج البحث

فلترة النتائج

Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing.

ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples.

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique.

The 16S rRNA gene-based PCR method used for the detection of segmented filamentous bacteria in the intestinal microbiota generates false-positive results.

A New Primer to Amplify pmoA Gene From NC10 Bacteria in the Sediments of Dongchang Lake and Dongping Lake.

Novel micelle PCR-based method for accurate, sensitive and quantitative microbiota profiling.

Broad-Range Detection of Microorganisms Directly from Bronchoalveolar Lavage Specimens by PCR/Electrospray Ionization-Mass Spectrometry.

Improved PCR primers to amplify 16S rRNA genes from NC10 bacteria.

Decontamination of 16S rRNA gene amplicon sequence datasets based on bacterial load assessment by qPCR.

Comparative analysis of two 16S rRNA gene-based PCR primer sets provides insight into the diversity distribution patterns of anammox bacteria in different environments.

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