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Quick and affordable DNA cloning by reconstitution of Seamless Ligation Cloning Extract using defined factors.

• Authors : Liu AY; Laboratory of RNA Systems, Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan.; Koga H

Subjects: DNA*/DNA*/DNA*/genetics ; Escherichia coli*/Escherichia coli*/Escherichia coli*/genetics; Cloning, Molecular

• Source: Genes to cells : devoted to molecular & cellular mechanisms [Genes Cells] 2023 Aug; Vol. 28 (8), pp. 553-562. Date of Electronic Publication: 2023 May 03.Publisher: Blackwell Science Ltd Country of Publication: England NLM ID: 9607379 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli.

• Authors : Shimojo M; Laboratory for Cell-Free Protein Synthesis, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, 565-0874, Japan.; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.

Subjects: Transcription, Genetic*; Bacterial Proteins/Bacterial Proteins/Bacterial Proteins/*metabolism ; Escherichia coli/Escherichia coli/Escherichia coli/*metabolism

• Source: Communications biology [Commun Biol] 2020 Mar 25; Vol. 3 (1), pp. 142. Date of Electronic Publication: 2020 Mar 25.Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN:

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Reconstitution in Proteoliposomes of the Recombinant Human Riboflavin Transporter 2 (SLC52A2) Overexpressed in E. coli .

• Authors : Console L; Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Via P. Bucci 4C, 87036 Arcavacata di Rende, Italy.; Tolomeo M

Subjects: Escherichia coli/Escherichia coli/Escherichia coli/*genetics ; Escherichia coli/Escherichia coli/Escherichia coli/*metabolism ; Membrane Transport Proteins/Membrane Transport Proteins/Membrane Transport Proteins/*genetics

• Source: International journal of molecular sciences [Int J Mol Sci] 2019 Sep 08; Vol. 20 (18). Date of Electronic Publication: 2019 Sep 08.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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Bacterial Heterologous Expression System for Reconstitution of Chloroplast Inner Division Ring and Evaluation of Its Contributors.

• Authors : Irieda H; Academic Assembly, Institute of Agriculture, Shinshu University, Nagano 399-4598, Japan. .; Shiomi D

Subjects: Transgenes*; Chloroplast Proteins/Chloroplast Proteins/Chloroplast Proteins/*genetics ; Chloroplasts/Chloroplasts/Chloroplasts/*metabolism

• Source: International journal of molecular sciences [Int J Mol Sci] 2018 Feb 11; Vol. 19 (2). Date of Electronic Publication: 2018 Feb 11.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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Rational design strategies for functional reconstitution of plant cytochrome P450s in microbial systems

• Authors : Zhou, Anqi; Zhou, Kang; Li, Yanran

Subjects: Biological Sciences; Industrial Biotechnology; Cytochrome P-450 Enzyme System

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Exponential propagation of large circular DNA by reconstitution of a chromosome-replication cycle.

• Authors : Su'etsugu M; Department of Life Science, College of Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo, 171-8501, Japan.; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan.

Subjects: DNA Replication/DNA Replication/DNA Replication/*genetics ; DNA, Circular/DNA, Circular/DNA, Circular/*chemical synthesis ; Escherichia coli/Escherichia coli/Escherichia coli/*genetics

• Source: Nucleic acids research [Nucleic Acids Res] 2017 Nov 16; Vol. 45 (20), pp. 11525-11534.Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN:

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Heterologous Expression of Recombinant Human Cytochrome P450 (CYP) in Escherichia coli : N-Terminal Modification, Expression, Isolation, Purification, and Reconstitution.

• Authors : Shang, Tao¹ (AUTHOR); Fang, Chee Mun¹ (AUTHOR); Ong, Chin Eng² (AUTHOR)

• Source: BioTech. Mar2023, Vol. 12 Issue 1, p17. 18p.

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Reconstitution of Protein Translation of Mycobacterium Reveals Functional Conservation and Divergence with the Gram-Negative Bacterium Escherichia coli.

• Authors : Srivastava A; New England Biolabs, Inc., 240 County Road, Ipswich, MA, 01938, United States of America.; Asahara H

Subjects: Escherichia coli/Escherichia coli/Escherichia coli/*metabolism ; Mycobacterium/Mycobacterium/Mycobacterium/*metabolism; Amino Acyl-tRNA Synthetases/Amino Acyl-tRNA Synthetases/Amino Acyl-tRNA Synthetases/metabolism

• Source: PloS one [PLoS One] 2016 Aug 26; Vol. 11 (8), pp. e0162020. Date of Electronic Publication: 2016 Aug 26 (Print Publication: 2016).Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet

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Functional reconstitution of an unusual Firmicutes σ factor into a Gram-negative heterologous host.

• Authors : Davis MC; Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.; Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.

Subjects: Transcription, Genetic*; DNA-Directed RNA Polymerases/DNA-Directed RNA Polymerases/DNA-Directed RNA Polymerases/*metabolism ; Escherichia coli/Escherichia coli/Escherichia coli/*enzymology

• Source: Canadian journal of microbiology [Can J Microbiol] 2015 Nov; Vol. 61 (11), pp. 818-26. Date of Electronic Publication: 2015 Aug 18.Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 0372707 Publication Model: Print-Electronic Cited Medium: Internet

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In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins.

• Authors : Aoyama, Ryo^1,2 (AUTHOR); Masuda, Keiko¹ (AUTHOR); Shimojo, Masaru^1,2 (AUTHOR)

Subjects: *RECOMBINANT proteins; *ESCHERICHIA coli; *RIBOSOMES

• Source: Journal of Biochemistry. Feb2022, Vol. 171 Issue 2, p227-237. 11p.

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