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Academic Journal

Dynamic structure of E. coli cytoplasm: supramolecular complexes and cell aging impact spatial distribution and mobility of proteins.

  • Authors : Linnik D; Department of Biochemistry, University of Groningen, Groningen, Nijenborgh 4, 9747 AG, the Netherlands.; Maslov I

Subjects: Escherichia coli*/Escherichia coli*/Escherichia coli*/metabolism ; Escherichia coli*/Escherichia coli*/Escherichia coli*/genetics ; Escherichia coli*/Escherichia coli*/Escherichia coli*/drug effects

  • Source: Communications biology [Commun Biol] 2024 Apr 27; Vol. 7 (1), pp. 508. Date of Electronic Publication: 2024 Apr 27.Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN:

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Academic Journal

Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coli.

  • Authors : Tungekar AA; Protein and Structural Biology Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, 90220, Oulu, Finland.; Ruddock LW

Subjects: Escherichia coli*/Escherichia coli*/Escherichia coli*/genetics ; Single-Chain Antibodies*/Single-Chain Antibodies*/Single-Chain Antibodies*/genetics; Cytoplasm

  • Source: Scientific reports [Sci Rep] 2023 Aug 30; Vol. 13 (1), pp. 14188. Date of Electronic Publication: 2023 Aug 30.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN:

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Academic Journal

Efficient Production of Fc Fusion Proteins in the Cytoplasm of Escherichia coli : Dissecting and Mitigating Redox Heterogeneity.

  • Authors : Tungekar AA; Protein and Structural Biology Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, 90220 Oulu, Finland.; Ruddock LW

Subjects: Escherichia coli*/Escherichia coli*/Escherichia coli*/chemistry ; Escherichia coli Proteins*/Escherichia coli Proteins*/Escherichia coli Proteins*/genetics ; Escherichia coli Proteins*/Escherichia coli Proteins*/Escherichia coli Proteins*/chemistry

  • Source: International journal of molecular sciences [Int J Mol Sci] 2022 Nov 25; Vol. 23 (23). Date of Electronic Publication: 2022 Nov 25.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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Academic Journal

Effects of variable domain orientation on anti-HER2 single-chain variable fragment antibody expressed in the Escherichia coli cytoplasm.

  • Authors : Koçer İ; Department of Chemical Engineering, Hacettepe University, Ankara, Turkey.; Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, USA.

Subjects: Cytoplasm/Cytoplasm/Cytoplasm/*metabolism ; Escherichia coli/Escherichia coli/Escherichia coli/*metabolism ; Receptor, ErbB-2/Receptor, ErbB-2/Receptor, ErbB-2/*immunology

  • Source: Biotechnology progress [Biotechnol Prog] 2021 Mar; Vol. 37 (2), pp. e3102. Date of Electronic Publication: 2020 Nov 20.Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8506292 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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Academic Journal

Production of Extracellular Matrix Proteins in the Cytoplasm of E. coli : Making Giants in Tiny Factories.

  • Authors : Sohail AA; Faculty of Biochemistry and Molecular Medicine, University of Oulu, 90220 Oulu, Finland.; Gaikwad M

Subjects: Cytoplasm/Cytoplasm/Cytoplasm/*metabolism ; Escherichia coli/Escherichia coli/Escherichia coli/*metabolism ; Escherichia coli Proteins/Escherichia coli Proteins/Escherichia coli Proteins/*biosynthesis

  • Source: International journal of molecular sciences [Int J Mol Sci] 2020 Jan 21; Vol. 21 (3). Date of Electronic Publication: 2020 Jan 21.Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067

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Academic Journal

Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzyme.

  • Authors : Jalomo-Khayrova E; Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque Industrial Internacional, 22390, Tijuana, BCN, México.; Mares RE

Subjects: Cysteine Proteases/Cysteine Proteases/Cysteine Proteases/*genetics ; Cytoplasm/Cytoplasm/Cytoplasm/*metabolism ; Escherichia coli/Escherichia coli/Escherichia coli/*genetics

  • Source: BMC biotechnology [BMC Biotechnol] 2018 Apr 03; Vol. 18 (1), pp. 20. Date of Electronic Publication: 2018 Apr 03.Publisher: BioMed Central Country of Publication: England NLM ID: 101088663 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6750

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Academic Journal

Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media.

  • Authors : Gąciarz A; Faculty of Biochemistry and Molecular Medicine, University of Oulu, P.O. Box 5400, 90014, Oulu, Finland.; Khatri NK

Subjects: Cytoplasm/Cytoplasm/Cytoplasm/*chemistry ; Disulfides/Disulfides/Disulfides/*chemistry ; Escherichia coli/Escherichia coli/Escherichia coli/*genetics

  • Source: Microbial cell factories [Microb Cell Fact] 2017 Jun 15; Vol. 16 (1), pp. 108. Date of Electronic Publication: 2017 Jun 15.Publisher: BioMed Central Country of Publication: England NLM ID: 101139812 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2859

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Academic Journal

Stabilization of SecA ATPase by the primary cytoplasmic salt of Escherichia coli.

  • Authors : Roussel G; Department of Physiology and Biophysics, University of California, Irvine, Irvine, California, 92697-4560.; Lindner E

Subjects: Cytoplasm/Cytoplasm/Cytoplasm/*chemistry ; Escherichia coli/Escherichia coli/Escherichia coli/*metabolism ; Escherichia coli Proteins/Escherichia coli Proteins/Escherichia coli Proteins/*metabolism

  • Source: Protein science : a publication of the Protein Society [Protein Sci] 2019 Jun; Vol. 28 (6), pp. 984-989. Date of Electronic Publication: 2019 May 01.Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9211750 Publication Model: Print-Electronic Cited Medium:

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Academic Journal

A revolutionary approach facilitating co-expression of authentic human epidermal growth factor and basic fibroblast growth factor in both cytoplasm and culture medium of Escherichia coli.

  • Authors : Kwong KW; Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.; Wong WK

Subjects: Culture Media/Culture Media/Culture Media/*metabolism ; Cytoplasm/Cytoplasm/Cytoplasm/*metabolism ; Epidermal Growth Factor/Epidermal Growth Factor/Epidermal Growth Factor/*genetics

  • Source: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2013 Oct; Vol. 97 (20), pp. 9071-80. Date of Electronic Publication: 2013 Jul 24.Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN:

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Academic Journal

Systematic screening of soluble expression of antibody fragments in the cytoplasm of E. coli.

  • Authors : Gaciarz A; Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, Oulu, Finland. .; Veijola J

Subjects: Cytoplasm/Cytoplasm/Cytoplasm/*metabolism ; Escherichia coli/Escherichia coli/Escherichia coli/*metabolism ; Immunoglobulin Fragments/Immunoglobulin Fragments/Immunoglobulin Fragments/*metabolism

  • Source: Microbial cell factories [Microb Cell Fact] 2016 Jan 25; Vol. 15, pp. 22. Date of Electronic Publication: 2016 Jan 25.Publisher: BioMed Central Country of Publication: England NLM ID: 101139812 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2859

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