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Academic Journal

Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis.

  • Authors : Paclikova P; Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00, Brno, Czech Republic.; Harnos J

Subjects: Plasmids*/Plasmids*/Plasmids*/genetics ; Cloning, Molecular*/Cloning, Molecular*/Cloning, Molecular*/methods ; Mutagenesis, Site-Directed*/Mutagenesis, Site-Directed*/Mutagenesis, Site-Directed*/methods

  • Source: Scientific reports [Sci Rep] 2024 Sep 16; Vol. 14 (1), pp. 21591. Date of Electronic Publication: 2024 Sep 16.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Golden EGG, a simplified Golden Gate cloning system to assemble multiple fragments.

  • Authors : Biró JB; Institute of Plant Biology, HUN-REN Biological Research Centre Szeged, Szeged, Hungary.; Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary.

Subjects: Cloning, Molecular*/Cloning, Molecular*/Cloning, Molecular*/methods ; Genetic Vectors*/Genetic Vectors*/Genetic Vectors*/genetics; DNA/DNA/DNA/genetics

  • Source: Scientific reports [Sci Rep] 2024 Oct 25; Vol. 14 (1), pp. 25288. Date of Electronic Publication: 2024 Oct 25.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Cloning and functional characterization of the legumin A gene (EuLEGA) from Eucommia ulmoides Oliver.

  • Authors : Zheng L; The Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.; Zhao DG

Subjects: Cloning, Molecular* ; Plants, Genetically Modified*/Plants, Genetically Modified*/Plants, Genetically Modified*/genetics ; Eucommiaceae*/Eucommiaceae*/Eucommiaceae*/genetics

  • Source: Scientific reports [Sci Rep] 2024 Jun 19; Vol. 14 (1), pp. 14111. Date of Electronic Publication: 2024 Jun 19.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Cloning and functional characterization of the peptide deformylase encoding gene EuPDF1B from Eucommia ulmoides Oliv.

  • Authors : Wang Y; Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, China.; Zhao DG

Subjects: Eucommiaceae*/Eucommiaceae*/Eucommiaceae*/genetics ; Eucommiaceae*/Eucommiaceae*/Eucommiaceae*/metabolism ; Plants, Genetically Modified*/Plants, Genetically Modified*/Plants, Genetically Modified*/genetics

  • Source: Scientific reports [Sci Rep] 2024 May 21; Vol. 14 (1), pp. 11587. Date of Electronic Publication: 2024 May 21.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Cloning of the Arabidopsis SMAP2 promoter and analysis of its expression activity.

  • Authors : Bao A; College of Life Sciences, Jilin Agricultural University, Changchun, 130118, People's Republic of China.; Jiao T

Subjects: Promoter Regions, Genetic* ; Arabidopsis*/Arabidopsis*/Arabidopsis*/genetics ; Arabidopsis Proteins*/Arabidopsis Proteins*/Arabidopsis Proteins*/genetics

  • Source: Scientific reports [Sci Rep] 2024 May 20; Vol. 14 (1), pp. 11451. Date of Electronic Publication: 2024 May 20.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Improved surrogate eggshell incubation system for assisted reproductive technologies such as cloning, genome modification, and de-extinction efforts.

  • Authors : Yang H; Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, 55365, Republic of Korea.; Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.

Subjects: Chickens* ; Egg Shell*; Animals

  • Source: Scientific reports [Sci Rep] 2024 Sep 05; Vol. 14 (1), pp. 20796. Date of Electronic Publication: 2024 Sep 05.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Cloning, characterization, and spatio-temporal expression patterns of HdhSPARC and its responses to multiple stressors.

  • Authors : Hanif MA; Department of Fisheries Science, Chonnam National University, Yeosu, 59626, South Korea.; Hossen S

Subjects: Hydrogen Peroxide*/Hydrogen Peroxide*/Hydrogen Peroxide*/metabolism ; Gastropoda*/Gastropoda*/Gastropoda*/genetics ; Gastropoda*/Gastropoda*/Gastropoda*/metabolism

  • Source: Scientific reports [Sci Rep] 2024 Jan 26; Vol. 14 (1), pp. 2224. Date of Electronic Publication: 2024 Jan 26.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Molecular cloning, subcellular localization, and rapid recruitment to DNA damage sites of chicken Ku70.

  • Authors : Koike M; Institute for Quantum Medical Science, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba, 263-8555, Japan. .; Life Science Course, Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama, Saitama, 338-8570, Japan. .

Subjects: Antigens, Nuclear*/Antigens, Nuclear*/Antigens, Nuclear*/metabolism ; Chickens*/Chickens*/Chickens*/genetics ; Chickens*/Chickens*/Chickens*/metabolism

  • Source: Scientific reports [Sci Rep] 2024 Jan 12; Vol. 14 (1), pp. 1188. Date of Electronic Publication: 2024 Jan 12.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Structure prediction, docking studies and molecular cloning of novel Pichia kudriavzevii YK46 metalloprotease (MetPr) for improvement of feather waste biodegradation.

  • Authors : Abd El-Aziz NM; Microbial Genetic Department, Biotechnology Research Institute, National Research Centre, 33 El Buhouth ST, Dokki, Cairo, 12622, Egypt. .; Khalil BE

Subjects: Feathers*/Feathers*/Feathers*/metabolism ; Escherichia coli*/Escherichia coli*/Escherichia coli*/genetics; Animals Pichia kudriavzevii

  • Source: Scientific reports [Sci Rep] 2023 Nov 15; Vol. 13 (1), pp. 19989. Date of Electronic Publication: 2023 Nov 15.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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Academic Journal

Cloning, computational analysis and expression profiling of steroid 5 alpha-reductase 1 (SRD5A1) gene during reproductive phases and ovatide stimulation in endangered catfish, Clarias magur.

  • Authors : Agarwal D; Institute of Fisheries Post Graduate Studies, TNJFU, Kazhipattur, India.; Kumar G

Subjects: Cholestenone 5 alpha-Reductase*/Cholestenone 5 alpha-Reductase*/Cholestenone 5 alpha-Reductase*/metabolism ; Catfishes*/Catfishes*/Catfishes*/genetics ; Catfishes*/Catfishes*/Catfishes*/metabolism

  • Source: Scientific reports [Sci Rep] 2023 Nov 09; Vol. 13 (1), pp. 19553. Date of Electronic Publication: 2023 Nov 09.Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited

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