نتائج البحث

Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections

• Authors : Andersen, Marissa Jeme; Fong, ChunKi; La Bella, Alyssa Ann ...

• Source: eLife ; volume 11 ; ISSN 2050-084X

تفاصيل العنوان

Comment on ‘SARS-CoV-2 suppresses anticoagulant and fibrinolytic gene expression in the lung’

• Authors : FitzGerald, Ethan S; Jamieson, Amanda M;

• Source: eLife ; volume 11 ; ISSN 2050-084X

تفاصيل العنوان

Author response: Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis

• Authors : McPherson, Helen R; Duval, Cedric; Baker, Stephen R

تفاصيل العنوان

Decision letter: Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis

• Authors : Iqbal, Jameel; Zaidi, Mone

تفاصيل العنوان

Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis

• Authors : McPherson, Helen R; Duval, Cedric; Baker, Stephen R ...

• Source: eLife ; volume 10 ; ISSN 2050-084X

تفاصيل العنوان

Inference from longitudinal laboratory tests characterizes temporal evolution of COVID-19-associated coagulopathy (CAC)

• Authors : Pawlowski, Colin; Wagner, Tyler; Puranik, Arjun

• Source: eLife ; volume 9 ; ISSN 2050-084X

تفاصيل العنوان

Figure 10. G6b knockout megakaryocytes show enhanced spreading on perlecan. ; Adhesion of WT and Mpig6b–/–MKs on perlecan. (i) Mean surface area of MKs was quantified with ImageJ. n = 4–6 mice/condition/genotype from three independent experiments; total cell numbers analyzed per condition/genotype were 77–188 for conditions with perlecan only and 1671–2866 for conditions with fibrinogen. P values were calculated using two-way ANOVA with Sidak’s post-hoc test, ***, p

تفاصيل العنوان

Figure 8. Heparan sulfate removal of perlecan facilitates platelet adhesion. ; The indicated substrates were coated alone or in combination onto wells in 96-well plates (2.5 µg/ml collagen and 10 µg/ml for all other substrates) overnight. Where indicated, wells were treated with 5 mU/ml heparinase III. Platelets from (A) humans or (B) mice were allowed to adhere for 1 hr and adhesion was quantified colorimetrically with 4-nitrophenyl phosphate(pNPP). (A) Human, platelets; n = 4–5 individual donors from 3 to 4 independent experiments; P-values were calculated using one-way ANOVA with Sidak’s post-hoc test. (B) Mouse platelets; n = 4 samples/condition/genotype from two independent experiments. Owing to severe thrombocytopenia, platelets from up to five mice were pooled for one KO sample. P-values for differences between WT and Mpig6b–/–mice were calculated using two-way ANOVA with Sidak’s post-hoc test. (C, D) Adhesion of (C) human or (D) WT and G6b–/–platelets on fibrinogen and perlecan. (i) Mean surface area of individual platelets quantified by KNIME software analysis. In panel (C) (i) n = 5 donors from two independent experiments. P-values were calculated using one-way ANOVA with Sidak’s post-hoc test. Total number of cells analyzed: fibrinogen, 1957; perlecan, 239; perlecan + heparinase III, 686. In panel (D) (i) n = 5–7 mice/condition/genotype from 2 to 3 independent experiments. P-values were calculated using two-way ANOVA with Sidak’s post-hoc test. Total number of cells analyzed: 134–176 for perlecan conditions, and 913–1277 for fibrinogen conditions. *, p

تفاصيل العنوان

Appendix 1—figure 9. Prediction examples. ; Per-residue PScores used to calculate the final full sequence PScore are shown for a selection of human proteins, with residues colored from purple (PScore ≤ −2) to white (PScore = 0) to green (PScore ≥4.0). Black triangles denote residues annotated by PhosphoSitePlus as targets of PTMs, blue triangles denote modification sites with known regulatory significance, and red circles denote modification sites with known disease relevance. Proteins are annotated with the percentage of GO terms (with at least 10 human proteins) and high PScore-enriched GO terms (Panther analysis, PScore ≥4, with O/E > 1) of which the protein is a member, as well as the total number of each for which the annotated protein has the highest PScore in the set. Examples are grouped by (A), involvement in synaptic plasticity and neuronal behavior, showing synaptic functional regulator FMR1, and synaptophysin; (B), intracellular biomaterials and related structural proteins, showing focal adhesion kinase 1, vimentin, and keratin type I cytoskeletal 10; (C), proteins involved in signaling pathways, showing CCR4-NOT transcription complex subunit 3, β-catenin, vitamin D3 receptor, and Smoothened homolog; and (D), proteins involved in extracellular biomaterials, showing fibrinogen alpha chain and dentin sialophosphoprotein. (E) The cystic fibrosis transmembrane conductance regulator is shown as an example of a negative prediction, even though containing a large region of intrinsic disorder (residues ~650–840).

تفاصيل العنوان

Author response image 1. ERp5 triggers αIIbβ3 integrin de-adhesion from fibrin. ; Platelet adhesion to immobilized fibrin in the absence of presence of 2 µM ERp5 was measured. Fibrin was immobilized in two ways: immobilized fibrinogen was incubated with thrombin and residual thrombin inactivated with hirudin (fbg/IIa), or pre-formed fibrin was directly immobilized (fibrin). Adhesion was measured in the first 4 minutes at a fluid shear rate of 1000 s-1. The bars and errors (1 SD) are from three measurements each from three different healthy donor platelets. *, p < 0.05, **, p < 0.01; assessed by unpaired, two-tailed Student’s t-test. Additional experimental details are as described in the manuscript for platelet adhesion to fibrinogen.

تفاصيل العنوان