Scale-up of Preparative Liquid Chromatography for Purification of Macrolide Antibiotics

A method was proposed for measuring the parameters of the adsorption isotherm and mass-transfer coefficients using an analytical column in order to simulate chromatograms of preparative liquid chromatography (prep LC). Close agreement between measured and simulated chromatograms was obtained for prep LC purification of erythromycin. The results showed that this method can be fully applied for scale-up from analytical LC to prep LC.