Glyoxal fixation: an immunohistochemistry assay evaluation.

Publisher: Historical Metallurgy Society Country of Publication: England NLM ID: 8003807 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2046-0236 (Electronic) Linking ISSN: 01478885 NLM ISO Abbreviation: J Histotechnol Subsets: MEDLINE

Publication: 2020- : Bedfordshire : Historical Metallurgy Society
Original Publication: [Downers Grove, IL : Mayo Publications]

Formaldehyde*/pharmacology ; Glyoxal*/pharmacology; Fixatives/pharmacology ; Humans ; Immunohistochemistry ; Tissue Fixation/methods

Analysis of surgical pathology specimens by histological techniques including immunohistochemistry (IHC) assays is a mainstay of disease diagnosis in humans. Neutral buffered formalin (NBF) is currently the primary fixative used, but its use is not without risks due to toxicity and carcinogenicity. Several glyoxal-based fixatives have been commercially produced, are considered safer alternatives to NBF, and produce histochemical staining results comparable to that of tissues fixed in NBF. However, previous studies evaluating IHC assay results in tissues fixed in NBF and glyoxal solutions have indicated mixed results. This study demonstrated that while tissues fixed in NBF were slightly superior to tissues fixed in glyoxal solutions among the 34 antibodies assayed with IHC, all fixative solutions produced results compatible for use in an anatomic pathology laboratory.

Keywords: FFPE; Glyoxal; IHC; NBF; fixative; formalin; immunohistochemistry

0 (Fixatives)
1HG84L3525 (Formaldehyde)
50NP6JJ975 (Glyoxal)

Date Created: 20211208 Date Completed: 20220525 Latest Revision: 20220706

20231215

10.1080/01478885.2021.1996125

34878359