Woody rootstock for efficient grafting of solanaceous vegetables and efficient grafting and seedling culture method thereof

11751,512

17/497992

October 11, 2021

The present application discloses a woody rootstock for efficient grafting of solanaceous vegetables and an efficient grafting and seedling culture method thereof. According to the present application, a woody rootstock clone with high consistency is provided through tissue culture, efficient grafting is completed through sleeve grafting technology, and the grafting survival rate is improved by regulating the healing environment. A new idea for efficient industrial grafting of solanaceous vegetables is provided, scions are imparted with new features through distant grafting, and the problems of low grafting efficiency and low survival rate are solved. The method has the advantages of strong operability, simplicity, high efficiency and low cost, and provides a technical support for the industrial production of grafted seedlings of solanaceous vegetables.

ZHEJIANG UNIVERSITY (Hangzhou, CN)

ZHEJIANG UNIVERSITY (Hangzhou, CN)

1. A method for efficiently grafting and seedling culture of solanaceous vegetables, comprising: (a) accelerating germination and sowing scion seeds of solanaceous vegetables; (b) after the scion seeds grow into seedlings, taking a portion above cotyledon and cutting lower section into a wedge shape on both sides, and inserting the lower section into a split of a rootstock, and adjusting a position of sleeve to fix scion on the rootstock; and (c) planting the grafted seedlings into matrix and proceeding to cultivation management, wherein the rootstock is obtained by: (1) taking newly induced branches of Lycium barbarum or Lycium ruthenicum Murr. and sterilizing; (2) cutting the branches into small stems, inoculating into a bud induction culture medium for bud induction germination, wherein in the bud induction culture medium, WPM (Woody Plant Medium) is used as the basic medium, and sucrose, agar, 6-BA, and NAA are added with concentrations of 30 g/L, 8 g/L, 1.0 mg/L and 0.5 mg/L respectively; the pH value of the bud induction culture medium is 5.8; cutting the branch into a small stem with an axillary bud, and inoculating into the bud induction medium, and culturing in an environment at 25° C. under a 12 h/12 h light/dark photoperiod with a light intensity of 4000 lux; (3) when the newly induced buds grow to 5 cm or more, cutting the buds into small stems, which are inoculated into a bud subculture propagation medium to establish a rootstock clone, wherein in the bud subculture propagation medium, WPM and MS in a volume ratio of 1:1 are used as a basic culture medium, sucrose and agar are added with the concentrations of 30 g/L and 8 g/L respectively, and the pH value of the bud subculture propagation medium is 5.8; (4) when the newly induced buds on the small stems grow to 3 cm or more, cutting at lower end horizontally, and inoculating into a rooting culture medium to make the buds grow into a complete plant, wherein in the rooting culture medium, a culture medium consisting of WPM and MS in a volume ratio of 1:1 are used as a basic culture medium, and sucrose, agar, IBA, NAA are added with the concentrations of 30 g/L, 8 g/L, 0.4 mg/L and 0.05 mg/L, respectively, and the pH value of the rooting culture medium is 5.8; (5) transplanting the complete plant into matrix for domestication; and (6) cutting off top end of the plant horizontally, removing leaves in an area of 2 cm below the flat cut, splitting the cut by 0.5 cm-0.8 cm to form a split, and sleeving the split, thus obtaining the rootstock which can be used for efficiently grafting solanaceous vegetables.

2. The method according to claim 1 , wherein in the step (1), growing a newly sprouted branches to a length of 5 cm-15 cm.

3. The method according to claim 1 , wherein in the step (1), rinsing the branches with clean running water for 2 h and placing on an ultra-clean workbench, soaking and washing with a 75% ethanol aqueous solution for 30 s, rinsing with sterile water for 2-3 times each for 1 min; soaking and washing with a 0.1 wt % mercuric chloride aqueous solution for 5 min, rinsing with sterile water for 3-5 times each for 1 min; and absorbing residual water by sterile filter paper.

4. The method according to claim 1 , wherein in the step (3), when the axillary bud grows to have a length of 5 cm or more and a stem diameter of 2 mm-3 mm, cutting it into small stems with an axillary bud, which inoculating into the bud subculture propagation medium for propagation culture in an environment at 25° C. under a 12 h/12 h light/dark photoperiod with a light intensity of 4000 lux to establish a rootstock clone.

5. The method according to claim 1 , wherein in the step (5), moving a complete plant into a hole tray filled with wet matrix, and culturing in an environment at 95% relative humidity at 25° C./18° C. under a 16 h/8 h light/dark photoperiod with a light intensity of 8000 lux.

6. The method according to claim 1 , wherein in the step (a), the germination accelerating process comprises: soaking seeds in warm water of 55° C. for 1 h, naturally cooling, placing the seeds in a culture dish covered with clean and moist filter paper, and culturing at 25° C. without illumination; the sowing process comprises: taking out seeds at beginning of germination and transferring them to a hole tray filled with wet matrix, culturing in an environment at 80% relative humidity at 25° C./18° C. under a 16 h/8 h light/dark photoperiod with a light intensity of 8000 lux.

7. The method according to claim 1 , wherein in the step (b), grafting the scion when it growing to have three complete leaves and one young leaf, and culturing in an environment at 95% relative humidity at 25° C./18° C. under a 16 h/8 h light/dark photoperiod with a light intensity of 8000 lux.

8. The method according to claim 1 , wherein in the step (c), gradually reducing the humidity to 60% after planting for 1 week-2 weeks.

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